Aberrant transmembrane signal transduction in Dictyostelium cells expressing a mutated ras gene

Dictyostelium discoideum cells contain a single ras gene (Dd-ras) that is highly homologous to mammalian ras genes. Cell transformation with a vector carrying a ras gene with a (glycine → threonine) missense mutation at position 12 causes an altered morphogenesis. Extracellular cAMP signals regulate...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-07, Vol.84 (14), p.4905-4909
Main Authors: Haastert, P.J.M. van, Kesbeke, F, Reymond, C.D, Firtel, R.A, Luderus, E, Driel, R. van
Format: Article
Language:English
Subjects:
Adenylyl Cyclases - metabolism
Aggregation
B lymphocytes
Biochemistry. Physiology. Immunology. Molecular biology
BIOLOGIA
Biological and medical sciences
BIOLOGIE
BIOLOGY
Cell aggregates
Cells
CHAMPIGNON
Chemotaxis
Cyclic AMP - physiology
Cyclic GMP - physiology
DESENSITIZATION
Dictyostelium - genetics
Dictyostelium - physiology
DICTYOSTELIUM DISCOIDERUM
Enzyme Activation
Fundamental and applied biological sciences. Psychology
Fungal Proteins - genetics
Fungal Proteins - physiology
FUNGI
GENE
Gene expression regulation
GENES
Guanylate Cyclase - metabolism
Inurement
MOHO
MOISISSURE
Morphogenesis
MOULDS
MUTACION
MUTATION
Oncogenes
Protozoa
ras genes
ras Proteins
Signal transduction
TAXIE
TAXIS
Transformation, Genetic
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Summary:Dictyostelium discoideum cells contain a single ras gene (Dd-ras) that is highly homologous to mammalian ras genes. Cell transformation with a vector carrying a ras gene with a (glycine → threonine) missense mutation at position 12 causes an altered morphogenesis. Extracellular cAMP signals regulate morphogenesis and induce chemotaxis and the activation and subsequent desensitization of adenylate and guanylate cyclase. cAMP signal transduction was investigated in Dd-ras-transformed cells. Transformants that overexpress the mutated Dd-ras-Thr12 gene show normal activation and desensitization of adenylate cyclase and normal activation of guanylate cyclase. However, cAMP induces a stronger desensitization of guanylate cyclase stimulation in the Dd-ras-Thr12 transformant than in transformants overexpressing the Dd-ras-Gly12 wild-type gene or in untransformed cells. This effect was correlated with a reduced chemotactic sensitivity of the transformant expressing the mutated Dd-ras-Thr12 gene.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.14.4905