Enterically Transmitted non-A, non-B Hepatitis: Serial Passage of Disease in Cynomolgus Macaques and Tamarins and Recovery of Disease-Associated 27- to 34-nm Viruslike Particles

An experimental model of enterically transmitted non-A, non-B hepatitis (ET-NANBH) was established in tamarins (Saguinus mystax mystax) and cynomolgus macaques (Macaca fascicularis). First-passage animals were inoculated with two different stool suspensions obtained from human patients with well-def...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-09, Vol.84 (17), p.6277-6281
Main Authors: Bradley, Daniel W., Krawczynski, Krzysztof, Cook, E. H., McCaustland, Karen A., Humphrey, Charles D., Spelbring, John E., Myint, Hla, Maynard, James E.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Biological and medical sciences
Callitrichinae
Disease Models, Animal
Enzymes
Epidemiology
Feces - microbiology
Fundamental and applied biological sciences. Psychology
Hepatitis C - microbiology
Hepatitis C - pathology
Hepatitis C - transmission
Hepatitis E
Hepatitis Viruses - isolation & purification
Hepatitis Viruses - ultrastructure
Hepatitis, Viral, Human - transmission
Humans
Inoculation
Liver
Liver - pathology
Macaca fascicularis
Microbiology
Primates
Specimens
Virology
Viruses
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Summary:An experimental model of enterically transmitted non-A, non-B hepatitis (ET-NANBH) was established in tamarins (Saguinus mystax mystax) and cynomolgus macaques (Macaca fascicularis). First-passage animals were inoculated with two different stool suspensions obtained from human patients with well-defined ET-NANBH that originated from Burma and Pakistan, where epidemics of ET-NANBH occur. Both inocula contained 27- to 34-nm-diameter viruslike particles (VLPs) that were specifically aggregated by acute-phase ET-NANBH sera. ET-NANBH was subpassaged in both tamarins and cynomolgus macaques by using pools of stool suspensions from first-passage animals. One additional passage of disease in cynomolgus macaques resulted in a significantly shortened incubation period and increased severity of disease. VLPs similar to those found in the human inocula were observed in stool specimens of first-, second-, and third-passage cynomolgus macaques and in first- and second-passage tamarins. Our findings indicate that cynomolgus macaques are particularly suitable experimental models for studies of human ET-NANBH. The 27- to 34-nm VLPs found in infected human and primate stools appear to be etiologically linked to disease.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.17.6277