Autogenous Control of the S10 Ribosomal Protein Operon of Escherichia coli: Genetic Dissection of Transcriptional and Posttranscriptional Regulation

The S10 ribosomal protein operon is regulated autogenously by the product of one of the genes of the operon, the gene encoding ribosomal protein L4. We have used site-directed mutagenesis to isolate leader mutations affecting L4 control. The phenotypes of these mutants demonstrate that L4 regulates...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-09, Vol.84 (18), p.6516-6520
Main Authors: Freedman, Leonard P., Zengel, Janice M., Archer, Richard H., Lindahl, Lasse
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genes
Genetic mutation
Genetics
Messenger RNA
Microbiology
Mutation
Nucleic Acid Conformation
Operator regions
Operon
Operons
Plasmids
Protein Biosynthesis
Protein synthesis
Ribosomal Proteins - genetics
RNA Processing, Post-Transcriptional
RNA, Bacterial - genetics
RNA, Messenger - genetics
Terminator regions
Transcription, Genetic
Transcriptional regulatory elements
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Summary:The S10 ribosomal protein operon is regulated autogenously by the product of one of the genes of the operon, the gene encoding ribosomal protein L4. We have used site-directed mutagenesis to isolate leader mutations affecting L4 control. The phenotypes of these mutants demonstrate that L4 regulates both transcription and translation of the S10 operon. Several mutations abolish both levels of L4 control; others eliminate either transcriptional or translational control with little or no effect on the other mode of regulation. We conclude that L4-mediated transcriptional and translational control share some sequence requirements, but the two regulatory processes recognize somewhat different features of the S10 leader. Primary as well as secondary structures within the S10 leader appear to be involved.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.18.6516