c-src Gene Product in Developing Rat Brain is Enriched in Nerve Growth Cone Membranes

Differentiating rat neurons express high levels of the protooncogene product pp60c-src, a 60-kDa tyrosine kinase of unknown function encoded by c-src. pp60c-src was found to be concentrated at least 9-fold in membranes from a subcellular fraction of nerve growth cones, the motile tips of outgrowing...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-07, Vol.85 (14), p.5001-5005
Main Authors: Maness, Patricia F., Aubry, Muriel, Shores, Carol G., Frame, Lynn, Pfenninger, Karl H.
Format: Article
Language:English
Subjects:
Animals
Antigens
Axons - analysis
Brain
Brain - embryology
Brain - metabolism
Brain - ultrastructure
Cell Fractionation
Cell lines
Cell Membrane - analysis
Cellular immunity
Centrifugation, Density Gradient
Chick Embryo
Electrophoresis, Polyacrylamide Gel
Epithelial cells
Fluorescent Antibody Technique
Growth cones
Immunoassay
Nerves
Neurites
Neurons
Neurons - analysis
P branes
Protein Kinases
Protein-Tyrosine Kinases - metabolism
Proto-Oncogene Proteins - analysis
Proto-Oncogene Proteins - metabolism
Proto-Oncogene Proteins pp60(c-src)
Rats
Retina - analysis
Retina - embryology
Synaptosomes - analysis
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Summary:Differentiating rat neurons express high levels of the protooncogene product pp60c-src, a 60-kDa tyrosine kinase of unknown function encoded by c-src. pp60c-src was found to be concentrated at least 9-fold in membranes from a subcellular fraction of nerve growth cones, the motile tips of outgrowing neuronal processes. Indirect immunofluorescence staining of cultured chick retinal explants showed pp60c-src in neuronal growth cones and processes, with the antigen particularly concentrated in growth cones of long neurites. pp60c-src in growth cone membranes was an active tyrosine-specific protein kinase with elevated tyrosine-specific protein kinase activity and reduced electrophoretic mobility characteristic of the form of pp60c-src in central nervous system neurons. pp60c-src was present at lower levels in subcellular fractions from mature rat brain but synaptosomal membranes were not enriched. Preferential localization of an active form of pp60c-src in nerve growth cone membranes and persistence of pp60c-src in mature neurons suggest that this tyrosine kinase is important in growth conemediated neurite extension and synaptic plasticity.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.14.5001