Activation of the Fructose 1,6-bisphosphatase Gene by 1,25-dihydroxyvitamin D3 during Monocytic Differentiation

Cells from the human leukemia cell line HL-60 undergo terminal monocyte-like differentiation after exposure to either the active circulating form of vitamin D3, 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], or phorbol 12-myristate 13-acetate. Little is known about the genes that regulate monocytic differ...

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Published in:Proceedings of the National Academy of Sciences - PNAS 1988-09, Vol.85 (18), p.6904-6908
Main Authors: Solomon, David H., Raynal, Marie-Cecile, Tejwani, Gopi A., Cayre, Yvon E.
Format: Article
Language:English
Subjects:
Alveolar macrophages
Amino Acid Sequence
Amino acids
Base Sequence
Calcitriol - pharmacology
Cell lines
Clone Cells
Complementary DNA
DNA probes
Fructose-Bisphosphatase - genetics
Gels
Gene Expression Regulation
Humans
Leukemia, Myeloid, Acute - pathology
Liver cells
Messenger RNA
Molecular Sequence Data
Monocytes - drug effects
RNA
Tetradecanoylphorbol Acetate - pharmacology
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Summary:Cells from the human leukemia cell line HL-60 undergo terminal monocyte-like differentiation after exposure to either the active circulating form of vitamin D3, 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], or phorbol 12-myristate 13-acetate. Little is known about the genes that regulate monocytic differentiation. Using clonal variant cells of HL-60 origin, we constructed a cDNA library enriched for genes that are induced by 1,25-(OH)2D3. We now report that in HL-60, the fructose 1,6-bisphosphatase (FBPase; D-fructose-1,6-bisphosphate 1-phosphohydrolase, EC 3.1.3.11) gene is activated during 1,25-(OH)2D3-induced monocytic differentiation. This gene encodes two closely related mRNAs; one, activated by 1,25-(OH)2D3 at an early stage of HL-60 differentiation, encodes a protein that has homology to mammalian FBPase, a key enzyme in gluconeogenesis, although it does not exhibit its classical enzymatic activity. A second mRNA is activated by 1,25-(OH)2D3 mainly in peripheral blood monocytes. This mRNA is present in kidney as a unique transcript and encodes a protein with FBPase activity. Our data also show that this FBPase-encoding mRNA can be activated during monocytic maturation since it was detected in human alveolar macrophages.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.18.6904