Glutathione S-Transferase Ya Subunit Gene: Identification of Regulatory Elements Required for Basal Level and Inducible Expression

• Published in: Proceedings of the National Academy of Sciences - PNAS 1988-02, Vol.85 (4), p.1000-1004
• Main Authors: Telakowski-Hopkins, Claudia A., King, Ron G., Pickett, Cecil B.
• Format: Article
• Language: English
• Subjects: Animals; Aryl hydrocarbon receptors; Benzoflavones - pharmacology; beta-Naphthoflavone; Biological and medical sciences; Cell lines; Drug regulation; Enzyme Induction - drug effects; Fundamental and applied biological sciences. Psychology; Genes; Genes. Genome; Glutathione Transferase - biosynthesis; Glutathione Transferase - genetics; Hep G2 cells; Hepatocellular carcinoma; Humans; Liver Neoplasms, Experimental; Messenger RNA; Mice; Molecular and cellular biology; Molecular genetics; Plasmids; Promoter Regions, Genetic; Rats; Receptors, Aryl Hydrocarbon; Receptors, Drug - drug effects; Recombinant Fusion Proteins - biosynthesis; Regulatory Sequences, Nucleic Acid; RNA; Transfection; Tumor Cells, Cultured
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.85.4.1000

The function of the 5′-flanking region of a rat glutathione S-transferase Ya subunit structural gene has been examined in homologous and heterologous cells. By using the 5′-flanking region of the Ya subunit gene fused to the structural gene encoding chloramphenicol acetyltransferase, we have identified two cis-acting regulatory elements in the upstream region of the Ya gene. One element is required for maximum basal level expression in homologous cells, whereas the second element is required for inducible expression of the Ya gene by planar aromatic compounds such as β -naphthoflavone. The cis-acting element required for inducible expression of the Ya gene by β -naphthoflavone is functional only in cells with normal dioxin receptors.