Haplotype-Specific Differences in Signaling by Transfected Class II Molecules to a Ly-1+ B-Cell Clone

CH12.LX B cells are responsive to antigen-dependent differentiative signals transmitted through their surface Ek molecules. Although CH12.LX cells express surface Ak molecules with normal protein sequence, the Ak molecules do not deliver differentiative signals to these B cells. To determine whether...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1989-08, Vol.86 (15), p.5933-5937
Main Authors: Bishop, Gail Abendroth, Frelinger, Jeffrey A.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analysis of the immune response. Humoral and cellular immunity
Animals
Antibodies, Monoclonal
Antigens, Surface - analysis
B lymphocytes
B-Lymphocytes - immunology
Biological and medical sciences
Cell Differentiation
Cell interactions
Cell Line
Cell lines
Cells
Cellular differentiation
Cultured cells
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Haplotypes
Histocompatibility Antigens Class II - genetics
Histocompatibility Antigens Class II - immunology
Immunobiology
Molecular chains
Molecular Sequence Data
Molecules
Phenotype
Signal Transduction
Signals
T lymphocytes
Transfection
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Summary:CH12.LX B cells are responsive to antigen-dependent differentiative signals transmitted through their surface Ek molecules. Although CH12.LX cells express surface Ak molecules with normal protein sequence, the Ak molecules do not deliver differentiative signals to these B cells. To determine whether introduction of a new A molecule into CH12.LX cells would correct this deficiency, CH12.LX cells were transfected with the genes encoding new Aα and/or Aβ molecules. It was found that transfected cells responded to antigen-specific signals delivered via the EαkE$_{\beta}{}^{\text{k}}$, AαbA$_{\beta}{}^{\text{b}}$, or AαkA$_{\beta}{}^{\text{b}}$ molecule. However, the B cells did not respond to signals generated by the molecule AαkA$_{\beta}{}^{\text{k}}$, AαbA$_{\beta}{}^{\text{k}}$, AαkA$_{\beta}{}^{\text{d}}$, or AαkA$_{\beta}{}^{\text{u}}$. Comparison of these sequences suggests that two Aβ residues, His-47 and Trp-197, are important to the transmission of differentiative signals to B cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.15.5933