Regulation of a Bifunctional mRNA Results in Synthesis of Secreted and Nuclear Probasin

Probasin, a rat prostatic protein, is statistically related to members of a protein family that includes serum, cellular, and nuclear proteins. In vivo, probasin appears both in the secretions and in the nucleus of prostatic epithelial cells. Using primer extension and S1 nuclease protection assays...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1989-10, Vol.86 (20), p.7843-7847
Main Authors: Spence, Andrew M., Sheppard, Patricia C., Davie, James R., Matuo, Yuhsi, Nishi, Nozomu, McKeehan, Wallace L., Dodd, Janice G., Matusik, Robert J.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Androgen-Binding Protein - biosynthesis
Androgen-Binding Protein - genetics
Androgen-Binding Protein - metabolism
Animals
Base Sequence
Cell Nucleus - metabolism
Cloning, Molecular
Codon - genetics
Codons
Complementary DNA
Epithelial cells
Genes
Genes, Regulator
Immunoenzyme Techniques
Male
Messenger RNA
Molecular Sequence Data
Nuclear proteins
Nucleotides
Prostate
Prostate - metabolism
Protein Biosynthesis
Proteins
Rats
RNA, Messenger - genetics
Secretion
Sequence Homology, Nucleic Acid
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Summary:Probasin, a rat prostatic protein, is statistically related to members of a protein family that includes serum, cellular, and nuclear proteins. In vivo, probasin appears both in the secretions and in the nucleus of prostatic epithelial cells. Using primer extension and S1 nuclease protection assays we detected only one probasin mRNA. Thus, the localization of this protein to two separate cellular regions must be encoded by this one mRNA. Furthermore, in vitro translation of synthetic probasin mRNA demonstrated that a protein containing a signal peptide and a protein lacking a signal peptide were synthesized by initiation at different AUG codons. These data are consistent with a mechanism of translational regulation of a eukaryotic bifunctional mRNA.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.20.7843