Wegener Autoantigen and Myeloblastin are Encoded by a Single mRNA

• Published in: Proceedings of the National Academy of Sciences - PNAS 1991-10, Vol.88 (20), p.9253-9256
• Main Authors: Labbaye, Catherine, Musette, Philippe, Cayre, Yvon E.
• Format: Article
• Language: English
• Subjects: Antigenic determinants, haptens, artificial antigens; Antigens; Autoantigens; Autoantigens - genetics; Base Sequence; Biological and medical sciences; Blotting, Northern; Blotting, Southern; Cell growth; Cell Line; Cell lines; Complementary DNA; DNA Probes; DNA, Neoplasm - genetics; DNA, Neoplasm - isolation & purification; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Hematopoietic stem cells; Humans; Leukemia; Messenger RNA; Molecular immunology; Molecular Sequence Data; mRNA; Myeloblastin; Myeloid cells; Neutrophils; Oligodeoxyribonucleotides; Oligonucleotides, Antisense; Poly A - genetics; Poly A - isolation & purification; Polymerase Chain Reaction; proteinase 3; Restriction Mapping; RNA; RNA, Messenger - genetics; RNA, Messenger - isolation & purification; Serine Endopeptidases - genetics; Start codon
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.88.20.9253

Myeloblastin is a serine protease that has been identified in the human leukemia cell line HL-60. Downregulation of this protease can inhibit proliferation and induce differentiation of promyelocyte-like human leukemic cells. Proteinase 3, a serine protease of human neutrophils, has been identified as the Wegener autoantigen. A high level of homology between myeloblastin and proteinase 3 has suggested that they may be a single serine protease. We have recently completed the 5'-terminal nucleotide sequence of proteinase 3 and shown that its mRNA was also expressed in HL-60 cells and in cells from patients with acute myeloid leukemia. Here we demonstrate that myeloblastin and proteinase 3 are encoded by a single mRNA.