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Activity of the Agrobacterium T-DNA transfer machinery is affected by virB gene products

The oriT (origin of transfer) sequence and mob (mobilization) genes of plasmid RSF1010 can functionally replace transfer DNA (T-DNA) borders to generate an RSF1010 intermediate transferable to plants through activities of the tumor-inducing (Ti)-plasmid virulence (vir) genes of Agrobacterium tumefac...

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Published in:Proceedings of the National Academy of Sciences - PNAS 1991-10, Vol.88 (20), p.9350-9354
Main Authors: Ward, J.E. Jr. (University of Pennsylvania, Philadelphia, PA), Dale, E.M, Binns, A.N
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creator Ward, J.E. Jr. (University of Pennsylvania, Philadelphia, PA)
Dale, E.M
Binns, A.N
description The oriT (origin of transfer) sequence and mob (mobilization) genes of plasmid RSF1010 can functionally replace transfer DNA (T-DNA) borders to generate an RSF1010 intermediate transferable to plants through activities of the tumor-inducing (Ti)-plasmid virulence (vir) genes of Agrobacterium tumefaciens. Because the Ti plasmid virB gene products are hypothesized to form a membrane-localized T-DNA transport apparatus, we investigated whether specific virB genes were needed for RSF1010 transfer. Here we report that transformation of Nicotiana tabacum leaf explants by an RSF1010-derivative plasmid (pJW323) requires the essential virulence genes virB9, virB10, and virB11, consistent with the hypothesis that both the T-DNA and RSF1010 transfer intermediates utilize the same transport machinery. Further, while pJW323 is transferred into plant cells by Agrobacterium strains harboring both pJW323 and pTiA6, the initiation of crown gall tumors (i.e., T-DNA transfer) is greatly suppressed. Coordinate overexpression of the virB9, virB10, and virB11 gene products relieves pJW323-mediated oncogenic suppression and restores tumorigenicity, but does not increase the transfer frequency of pJW323 into plant cells. We propose that the virB9, virB10, and virB11 gene products function coordinately and stoichiometrically to enhance DNA transfer in a fashion specific for the T-DNA intermediate.
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subjects ADN
AGALLAS
Agrobacterium
AGROBACTERIUM TUMEFACIENS
Agronomy. Soil science and plant productions
Bacteria
Biological and medical sciences
DNA
EXPRESION GENICA
EXPRESSION DES GENES
FACTEUR IMMUNOLOGIQUE
FACTORES INMUNOLOGICOS
Fundamental and applied biological sciences. Psychology
GALLE
Gene expression
Genetic vectors
Molecular and cellular biology
Molecular genetics
NICOTIANA TABACUM
Plant cells
Plant tumor inducing plasmids
Plants
PLASMIDE
PLASMIDIOS
Plasmids
PODER PATOGENO
POUVOIR PATHOGENE
RESISTANCE AUX MALADIES
RESISTENCIA A LA ENFERMEDAD
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
Tumors
Virulence
title Activity of the Agrobacterium T-DNA transfer machinery is affected by virB gene products
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