UGA is Translated as Cysteine in Pheromone 3 of Euplotes Octocarinatus

Pheromone 3 mRNA of the ciliate Euplotes octocarinatus contains three in-frame UGA codons that are translated as cysteines. This was revealed from cDNA sequencing and from plasma desorption mass spectrometry of cleaved pheromone 3 in connection with pyridylethylation of the fragments. N-terminal seq...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1991-05, Vol.88 (9), p.3758-3761
Main Authors: MEYER, F, SCHMIDT, H. J, PLUÊMPER, E, HASILIK, A, MERSMANN, G, MEYER, H. E, ENGSTROM, A, HECKMANN, K
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Average linear density
Base Sequence
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Ciliophora - genetics
Ciliophora - physiology
Codons
Complementary DNA
Cysteine
DNA - genetics
Euplotes octocarinatus
Fundamental and applied biological sciences. Psychology
Genetic Code
Genetically modified organisms
Mass Spectrometry
Molecular Sequence Data
Peptide Chain Termination, Translational
Peptide Fragments - chemistry
Pheromones
Pheromones - genetics
Protozoa
Restriction Mapping
RNA
Sequencing
Stop codon
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Summary:Pheromone 3 mRNA of the ciliate Euplotes octocarinatus contains three in-frame UGA codons that are translated as cysteines. This was revealed from cDNA sequencing and from plasma desorption mass spectrometry of cleaved pheromone 3 in connection with pyridylethylation of the fragments. N-terminal sequence analysis of carboxymethylated protein confirmed this conclusion for the first of the three UGA codons. Besides UGA the common cysteine codons UGU and UGC are also used to encode cysteine. UAA functions as a termination codon. No UAG codon was found. In connection with results reported for other ciliates, this suggests that the role of the classic termination codons had not yet been established when the ciliates started to diverge from other eukaryotes.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.88.9.3758