Evidence for Regulatory Genes on Mouse Chromosome 7 that Affect the Quantitative Expression of Proteins in the Fetal and Newborn Liver

• Published in: Proceedings of the National Academy of Sciences - PNAS 1992-03, Vol.89 (6), p.2448-2452
• Main Authors: Giometti, Carol S., Gemmell, M. Anne, Taylor, John, Tollaksen, Sandra L., Angeletti, Ruth, Gluecksohn-Waelsch, Salome
• Format: Article
• Language: English
• Subjects: Albinism; Albinism - genetics; Animals; Animals, Newborn; Biological and medical sciences; Cellular biology; Chromosome Deletion; Chromosome Mapping; Chromosomes; Electrophoresis; Electrophoresis, Gel, Two-Dimensional; Enzymes; Enzymes - genetics; Fetus; Fundamental and applied biological sciences. Psychology; Gels; Genes; Genes, Regulator; Genes. Genome; Genetics; Government regulation; Homozygote; Homozygotes; Liver; Liver - embryology; Liver - physiology; Liver protein; Medical research; Mice; Mice, Inbred Strains - genetics; Molecular and cellular biology; Molecular genetics; Proteins; Proteins - genetics; Proteins - isolation & purification
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.89.6.2448

A series of deletions around the albino locus on mouse chromosome 7 is believed to include one or more regulatory genes that control the activities of a cluster of liver enzymes. To further characterize the functions of this region of the mouse genome, we have used quantitative two-dimensional electrophoresis to analyze the effects of two of these deletions, c3Hand c14CoS, on the expression of liver proteins. More than 400 distinct protein gene products were quantitated in livers from fetal and newborn wild-type homozygous (cch/cch), heterozygous (cch/c3Hor cch/c14CoS), and deletion homozygous (c3H/c3Hor c14CoS/c14CoS) mice. Livers of fetal and newborn c3Hheterozygotes and homozygous wild-type littermates produced qualitatively identical protein patterns after two-dimensional electrophoresis. In livers of c3Hhomozygous fetuses, however, abnormal amounts (either increased or decreased relative to homozygous wild-type and heterozygous littermates) of 29 proteins were found. Twenty-eight of these 29 protein anomalies were also found in livers of newborn c3Hhomozygotes. Livers of fetal and newborn mice homozygous for the c14CoSdeletion, which overlaps the c3Hdeletion and produces a similar phenotype, expressed normal amounts of these proteins. One of the 29 proteins (MSN807) has an amino-terminal sequence similar to a 23-kDa translationally controlled protein abundant in mouse erythroleukemia and sarcoma-180 cells. These results suggest that normal chromosome 7 contains genes, located within the region of the c3Hbut not the c14CoSdeletion, that regulate the abundance of specific proteins in the liver. These proteins cannot be related to the phenotypic alterations shared by the c3Hand c14CoSdeletions.