Isolation of a Candidate Human Hematopoietic Stem-Cell Population

We have identified a rare (0.05-0.1%) subset of human fetal bone marrow cells that contains multipotent hematopoietic precursors. The population of human precursor cells that express Thy-1 and CD34 but no known lineage markers is enriched for clonogenic activity that establishes long-term, multiline...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1992-04, Vol.89 (7), p.2804-2808
Main Authors: Baume, Charles M., Weissman, Irving L., Tsukamoto, Ann S., Buckle, Ann-Marie, Peault, Bruno
Format: Article
Language:English
Subjects:
Animals
Antigens, CD - analysis
Antigens, CD34
Antigens, Surface - analysis
B lymphocytes
Biological and medical sciences
Bone Marrow Cells
Cell Differentiation
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
Cell Separation
Cells
Cells, Cultured
Cellular biology
Coculture techniques
Colony-Forming Units Assay
Cultured cells
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Hematopoiesis
Hematopoietic stem cells
Hematopoietic Stem Cells - cytology
HLA antigens
Humans
In Vitro Techniques
Lymphocytes - cytology
Medical research
Membrane Glycoproteins - analysis
Mice
Mice, SCID
Molecular and cellular biology
Multipotent stem cells
Progenitor cells
Stromal cells
T lymphocytes
Thy-1 Antigens
Thymus Gland - cytology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have identified a rare (0.05-0.1%) subset of human fetal bone marrow cells that contains multipotent hematopoietic precursors. The population of human precursor cells that express Thy-1 and CD34 but no known lineage markers is enriched for clonogenic activity that establishes long-term, multilineage (myelomonocytic and B lymphoid) cultures on mouse marrow stromal lines. Further, the Thy-1+CD34+subset that takes up little of the fluorescent mitochondrial dye rhodamine 123 contains virtually all the cells that establish long-term cultures. In human fetal thymus transplanted into SCID (severe combined immunodeficiency) mice, Thy-1+CD34+fetal bone marrow cells differentiate into T lymphocytes. In two of nine cases, allogeneic Thy-1+CD34+cells could engraft intact human fetal bone marrow grown in SCID mice, resulting in donor-derived myeloid and B cells. By extrapolation, the rare human Thy-1+Lin-CD34+cell population contains pluripotent hematopoietic progenitors; we propose that it is highly enriched for candidate hematopoietic stem cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.89.7.2804