Airway Epithelial Cells are the Site of Expression of a Mammalian Antimicrobial Peptide Gene

We previously reported the isolation and characterization of a broad-spectrum antimicrobial peptide from the bovine tracheal mucosa, which we called tracheal antimicrobial peptide (TAP). We now show the TAP gene is expressed throughout the adult conducting airway, from nasal to bronchiolar tissue, b...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1993-05, Vol.90 (10), p.4596-4600
Main Authors: Diamond, Gill, Jones, Douglas E., Bevins, Charles L.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Aminoacids, peptides. Hormones. Neuropeptides
Analytical, structural and metabolic biochemistry
Animals
Anti-Infective Agents
Antimicrobial Cationic Peptides
Antimicrobials
Base Sequence
Biochemistry
Biological and medical sciences
Blotting, Southern
Cattle
Cloning, Molecular
Epithelial cells
Epithelium
Exons
Fundamental and applied biological sciences. Psychology
Gene Expression
Genes
Genomics
In Situ Hybridization
Messenger RNA
Molecular Sequence Data
Mucosa
Oligodeoxyribonucleotides - chemistry
Oligonucleotide probes
Peptides - genetics
Proteins
Respiratory Physiological Phenomena
Respiratory system
RNA
RNA, Messenger - genetics
Ungulates
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Summary:We previously reported the isolation and characterization of a broad-spectrum antimicrobial peptide from the bovine tracheal mucosa, which we called tracheal antimicrobial peptide (TAP). We now show the TAP gene is expressed throughout the adult conducting airway, from nasal to bronchiolar tissue, but not in tissues other than airway mucosa, as determined by Northern blot analysis. In situ hybridization of airway sections localizes TAP mRNA to columnar cells of the pseudostratified epithelium. We report the structural organization of the TAP gene and show that TAP is a member of a large family of related sequences with high nucleotide identity in the 5'exon. The data support the hypothesis that antimicrobial peptides contribute to host defense of the respiratory tract.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.90.10.4596