Estrogen Modulates Expression of the Glycosyltransferases that Synthesize Sulfated Oligosaccharides on Lutropin

The glycoprotein hormone lutropin (LH) bears oligosaccharides terminating with the sequence SO4-4-GalNAcβ1,4GlcNAcβ1,2Manα. We have determined that estrogen actively modulates expression of the GalNAc- and sulfotransferases responsible for synthesis of sulfated oligosaccharides on LH α and β subunit...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1993-12, Vol.90 (23), p.11127-11131
Main Authors: Dharmesh, Shylaja M., Baenziger, Jacques U.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Biochemistry
Biological and medical sciences
Carbohydrate Sequence
Estrogens
Estrogens - pharmacology
Female
Fundamental and applied biological sciences. Psychology
General aspects. Hormone interactions. Hormone actions on several organ systems. Adaptive reactions
Glycoproteins
Glycosylation
Glycosyltransferases - metabolism
Hormones
Kidneys
Luteinizing Hormone - metabolism
Molecular Sequence Data
Monoclonal antibodies
N-Acetylgalactosaminyltransferases - metabolism
Oligosaccharides
Ovariectomy
Pituitary Gland - metabolism
Protein Processing, Post-Translational
Proteins
Rats
Rats, Sprague-Dawley
Receptors
Salivary glands
Sulfotransferases - metabolism
Time Factors
Vertebrates: endocrinology
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Summary:The glycoprotein hormone lutropin (LH) bears oligosaccharides terminating with the sequence SO4-4-GalNAcβ1,4GlcNAcβ1,2Manα. We have determined that estrogen actively modulates expression of the GalNAc- and sulfotransferases responsible for synthesis of sulfated oligosaccharides on LH α and β subunits. Consequently, terminal glycosylation of LH oligosaccharides with GalNAc-4-SO4is maintained when LH synthesis and secretion are markedly increased, as occurs during the midcycle surge and following ovariectomy. Maintenance of sulfated oligosaccharides on LH has important biologic consequences because LH circulatory half-life as well as biologic activity at the hormone receptor level are dramatically affected by glycosylation. To our knowledge, regulation of glycosyltransferase levels in response to specific stimuli has not been observed previously, further emphasizing the biologic significance of glycosylation for expression of LH bioactivity in vivo.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.90.23.11127