Extracellular Matrix-Dependent Tissue-Specific Gene Expression in Mammary Epithelial Cells Requires both Physical and Biochemical Signal Transduction

Extracellular matrix (ECM) profoundly influences the growth and differentiation of the mammary gland epithelium, both in culture and in vivo. Utilizing a clonal population of mouse mammary epithelial cells that absolutely requires an exogenous ECM for function, we developed a rapid assay to study si...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1994-12, Vol.91 (26), p.12378-12382
Main Authors: Roskelley, C. D., Desprez, P. Y., Bissell, M. J.
Format: Article
Language:English
Subjects:
551000 - Physiological Systems
ANIMAL TISSUES
ANIMALS
B lymphocytes
Basement membrane
BASIC BIOLOGICAL SCIENCES
Biochemistry
BODY
Caseins - metabolism
Cell Aggregation
CELL DIFFERENTIATION
Cell lines
Cell nucleus
Cells
Cells, Cultured
Cellular biology
CONTROL
Cultured cells
Epithelial cells
EPITHELIUM
Extracellular Matrix - physiology
Female
Gene Expression Regulation, Developmental
GENETIC CONTROL
Genetics
Genistein
GLANDS
GROWTH
In Vitro Techniques
Integrins
Integrins - physiology
Isoflavones - pharmacology
MAMMALS
MAMMARY GLANDS
Medical research
MICE
Milk protein
ORGANS
PEST CONTROL
Phosphotyrosine
Prolactin - pharmacology
Receptor Aggregation
RODENTS
Signal Transduction
Tetradecanoylphorbol Acetate - pharmacology
Time Factors
TISSUES
Tyrosine - analogs & derivatives
Tyrosine - metabolism
VERTEBRATES
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Extracellular matrix (ECM) profoundly influences the growth and differentiation of the mammary gland epithelium, both in culture and in vivo. Utilizing a clonal population of mouse mammary epithelial cells that absolutely requires an exogenous ECM for function, we developed a rapid assay to study signal transduction by ECM. Two components of the cellular response to a basement membrane overlay that result in the expression of the milk protein β-casein were defined. The first component of this response involves a rounding and clustering of the cells that can be physically mimicked by plating the cells on a nonadhesive substratum. The second component is biochemical in nature, and it is associated with β1integrin clustering and increased tyrosine phosphorylation. The second component is initiated in a morphology-independent manner, but the proper translation of this biochemical signal into a functional response requires cell rounding and cell clustering. Thus, physical and biochemical signal transduction events contribute to the ECM-dependent regulation of tissue-specific gene expression in mouse mammary epithelial cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.91.26.12378