A Staphylococcus aureus Autolysin that has an N-Acetylmuramoyl-L-Alanine Amidase Domain and an Endo-β-N-Acetylglucosaminidase Domain: Cloning, Sequence Analysis, and Characterization

The Tn551 insertion site of the autolysis-deficient Staphylococcus aureus mutant RUSAL2 was cloned and used to identify the autolysis gene atl in the parent strain, RN450. The open reading frame for atl was 3768 bp in length, encoding a deduced protein of 1256 amino acids and molecular size of 137,3...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1995-01, Vol.92 (1), p.285-289
Main Authors: Oshida, Tadahiro, Sugai, Motoyuki, Komatsuzawa, Hitoshi, Hong, Yeong-Man, Suginaka, Hidekazu, Tomasz, Alexander
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Autolysis
Bacteria
Base Sequence
Biology
Cell walls
Cloning, Molecular
DNA
DNA Transposable Elements
Electrophoresis, Polyacrylamide Gel
Enzymes
Escherichia coli
Gels
Genes
Genes, Bacterial
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase - biosynthesis
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase - genetics
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase - isolation & purification
Molecular Sequence Data
Molecular Weight
Mutagenesis, Insertional
Mutation
N-Acetylmuramoyl-L-alanine Amidase - biosynthesis
N-Acetylmuramoyl-L-alanine Amidase - genetics
N-Acetylmuramoyl-L-alanine Amidase - isolation & purification
Open Reading Frames
Plasmids
Recombinant Proteins - biosynthesis
Recombinant Proteins - isolation & purification
Restriction Mapping
Sequence Homology, Amino Acid
Sequencing
Staphylococcus aureus - enzymology
Staphylococcus aureus - genetics
Staphylococcus aureus - metabolism
Citations: Items that cite this one
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Description
Summary:The Tn551 insertion site of the autolysis-deficient Staphylococcus aureus mutant RUSAL2 was cloned and used to identify the autolysis gene atl in the parent strain, RN450. The open reading frame for atl was 3768 bp in length, encoding a deduced protein of 1256 amino acids and molecular size of 137,381 Da. The atl gene product is a bifunctional protein that has an amidase domain and an endo-β-N-acetylglucosaminidase domain which must undergo proteolytic processing to generate the two extracellular lytic enzymes found in the culture broth of S. aureus.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.92.1.285