Assignment of Rfp-Y to the chicken major histocompatibility complex/NOR microchromosome and evidence for high-frequency recombination associated with the nucleolar organizer region

Rfp-Y is a second region in the genome of the chicken containing major histocompatibility complex (MHC) class I and II genes. Haplotypes of Rfp-Y assort independently from haplotypes of the B system, a region known to function as a MHC and to be located on chromosome 16 (a microchromosome) with the...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1996-04, Vol.93 (9), p.3958-3962
Main Authors: Miller, M.M. (Beckman Research Institute of the City of Hope National Medical Center, Duarte, CA.), Goto, R.M, Taylor, R.L. Jr, Zoorob, R, Auffray, C, Briles, R.W, Briles, W.E, Bloom, S.E
Format: Article
Language:English
Subjects:
Animals
Birds
CARTE GENETIQUE
Chickens
CHROMOSOME
Chromosome Mapping
Cosmids
CROMOSOMAS
Crosses, Genetic
Diploidy
Female
GENE
GENES
Genes, MHC Class I
GENETICA
GENETIQUE
Genome
GENOTIPOS
GENOTYPE
Immunity (Disease)
LOCALISATION DE GENE
LOCALIZACION DE GENES
Major Histocompatibility Complex
Male
MAPAS GENETICOS
Multigene Family
NOMBRE CHROMOSOMIQUE
NUCLEOLE
NUCLEOLOS
Nucleolus Organizer Region
NUMERO DE CROMOSOMAS
POLLO
POULET
RECOMBINACION
RECOMBINAISON
Recombination, Genetic
SEGREGACION
SEGREGATION
SISTEMA MHC
SYSTEME MHC
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
Trisomy
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Summary:Rfp-Y is a second region in the genome of the chicken containing major histocompatibility complex (MHC) class I and II genes. Haplotypes of Rfp-Y assort independently from haplotypes of the B system, a region known to function as a MHC and to be located on chromosome 16 (a microchromosome) with the single nucleolar organizer region (NOR) in the chicken genome. Linkage mapping with reference populations failed to reveal the location of Rfp-Y, leaving Rfp-Y unlinked in a map containing 400 markers. A possible location of Rfp-Y became apparent in studies of chickens trisomic for chromosome 16 when it was noted that the intensity of restriction fragments associated with Rfp-Y increased with increasing copy number of chromosome 16. Further evidence that Rfp-Y might be located on chromosome 16 was obtained when individuals trisomic for chromosome 16 were found to transmit three Rfp-Y haplotypes. Finally, mapping of cosmic cluster III of the molecular map of chicken MHC genes (containing a MHC class II gene and two rRNA genes) to Rfp-Y validated the assignment of Rfp-Y to the MHC/NOR microchromosome. A genetic map can now be drawn for a portion of chicken chromosome 16 with Rfp-Y, encompassing two MHC class I and three MHC class II genes, separated from the B system by a region containing the NOR and exhibiting highly frequent recombination
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.93.9.3958