A Plant-specific Cyclin-dependent Kinase Is Involved in the Control of G2/M Progression in Plants

Cyclin-dependent kinases (CDKs) control the key transitions in the eukaryotic cell cycle. All the CDKs known to control G2/M progression in yeast and animals are distinguished by the characteristic PSTAIRE motif in their cyclin-binding domain and are closely related. Higher plants contain in additio...

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Bibliographic Details
Published in:The Journal of biological chemistry 2001-09, Vol.276 (39), p.36354-36360
Main Authors: Porceddu, Andrea, Stals, Hilde, Reichheld, Jean-Philippe, Segers, Gerda, De Veylder, Lieven, de Pinho Barrôco, Rosa, Casteels, Peter, Van Montagu, Marc, Inzé, Dirk, Mironov, Vladimir
Format: Article
Language:English
Subjects:
Amino Acid Motifs
Amino Acid Sequence
Arabidopsis Proteins
Binding, Competitive
Cell Cycle
Cell Nucleus
Cells, Cultured
Chromatography, Gel
Cyclin-Dependent Kinases - genetics
Cyclin-Dependent Kinases - physiology
Down-Regulation
Flow Cytometry
G2 Phase
Genes, Dominant
Histones - metabolism
Immunoblotting
Mitosis
Molecular Sequence Data
Mutation
Nicotiana - cytology
Plants, Genetically Modified - metabolism
Plants, Toxic
Plasmids - metabolism
Precipitin Tests
Protein Binding
Protein Kinases - metabolism
Protein Structure, Tertiary
Sequence Homology, Amino Acid
Time Factors
Transgenes
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Summary:Cyclin-dependent kinases (CDKs) control the key transitions in the eukaryotic cell cycle. All the CDKs known to control G2/M progression in yeast and animals are distinguished by the characteristic PSTAIRE motif in their cyclin-binding domain and are closely related. Higher plants contain in addition a number of more divergent non-PSTAIRE CDKs with still obscure functions. We show that a plant-specific type of non-PSTAIRE CDKs is involved in the control of the G2/M progression. In synchronized tobacco BY-2 cells, the corresponding protein, accumulated in a cell cycle-regulated fashion, peaking at the G2/M transition. The associated histone H1 kinase activity reached a maximum in mitosis and required a yet unidentified subunit to be fully active. Down-regulation of the associated kinase activity in transgenic tobacco plants using a dominant-negative mutation delayed G2/M transition. These results provide the first evidence that non-PSTAIRE CDKs are involved in the control of the G2/M progression in plants.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M011060200