The ATPase Reaction Cycle of Yeast DNA Topoisomerase II

• Published in: The Journal of biological chemistry 2001-07, Vol.276 (30), p.27893-27898
• Main Authors: Baird, Cheryl L., Gordon, Matthew S., Andrenyak, David M., Marecek, James F., Lindsley, Janet E.
• Format: Article
• Language: English
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M102544200

DNA topoisomerase II catalyzes the transport of one DNA duplex through a transient break in a second duplex using a complex ATP hydrolysis mechanism. Two key rates in the ATPase mechanism, ATP resynthesis and phosphate release, were investigated using 18 O exchange and stopped-flow phosphate release experiments, respectively. The 18 O exchange results showed that the rate of ATP resynthesis on the topoisomerase II active site was slow compared with the rate of phosphate release. When topoisomerase II was bound to DNA, phosphate was released slowly, with a lag. Since each of the preceding steps is known to occur rapidly, phosphate release is apparently a rate-determining step. The length of the lag phase was unaffected by etoposide, indicating that inhibiting DNA religation inhibits the ATPase reaction cycle at some step following phosphate release. By combining the 18 O exchange and phosphate release results, the rate constant for ATP resynthesis can be calculated as ∼0.5 s − 1 . These data support the mechanism of sequential hydrolysis of two ATP by DNA topoisomerase II.