Cloning and Characterization of a Novel Na+-dependent Glucose Transporter (NaGLT1) in Rat Kidney

To identify novel transporters in the kidney, we have constructed an mRNA data base composed of 1000 overall clones by random sequencing of a male rat kidney cDNA library. After a BLAST search, ∼40% of the clones were unknown and/or unannotated and were screened by measuring the uptake of various co...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 2003-04, Vol.278 (17), p.14669-14676
Main Authors: Horiba, Naoshi, Masuda, Satohiro, Takeuchi, Ayako, Takeuchi, Daisuke, Okuda, Masahiro, Inui, Ken-ichi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cloning, Molecular
Gene Library
Kidney - chemistry
Kidney - metabolism
Kinetics
Male
Membrane Glycoproteins
Molecular Sequence Data
Monosaccharide Transport Proteins - genetics
Monosaccharide Transport Proteins - metabolism
Rats - genetics
RNA, Messenger - metabolism
Sodium-Glucose Transporter 1
Sodium-Glucose Transporter 2
Substrate Specificity
Tissue Distribution
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To identify novel transporters in the kidney, we have constructed an mRNA data base composed of 1000 overall clones by random sequencing of a male rat kidney cDNA library. After a BLAST search, ∼40% of the clones were unknown and/or unannotated and were screened by measuring the uptake of various compounds usingXenopus oocytes. One clone stimulated the uptake of α-methyl-d-glucopyranoside and therefore was termed rat Na+-dependent glucose transporter 1 (rNaGLT1). The rNaGLT1 cDNA (2173 bp) has an open reading frame encoding a 484-amino acid protein, showing
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M212240200