ΔNp63α Expression Is Regulated by the Phosphoinositide 3-Kinase Pathway

• Published in: The Journal of biological chemistry 2003-12, Vol.278 (51), p.51408-51414
• Main Authors: Barbieri, Christopher E., Barton, Christopher E., Pietenpol, Jennifer A.
• Format: Article
• Language: English
• Subjects: Cell Line, Tumor; Cells, Cultured; DNA-Binding Proteins - biosynthesis; DNA-Binding Proteins - genetics; Enzyme Inhibitors - pharmacology; Epidermal Growth Factor - pharmacology; Gene Expression Regulation - drug effects; Humans; Keratinocytes - metabolism; Keratinocytes - pathology; Neoplasms, Squamous Cell - etiology; Phosphatidylinositol 3-Kinases - physiology; Phosphoinositide-3 Kinase Inhibitors; Protein Subunits - antagonists & inhibitors; Protein Subunits - physiology; RNA, Messenger - biosynthesis; RNA, Small Interfering - pharmacology
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M309943200

p63 is a homologue of p53 that functions to maintain progenitor cell populations in stratified epithelia. ΔNp63α is overexpressed in epithelial cancers and has been shown to have oncogenic properties. We have previously reported that inhibition of epidermal growth factor receptor signaling results in a decrease in ΔNp63α expression. Here, we demonstrate ΔNp63α is a target of the phosphoinositide-3-kinase (PI3K) pathway downstream of the epidermal growth factor receptor. Treatment of keratinocytes with epidermal growth factor results in an increase in ΔNp63α expression at the mRNA level, which is abrogated by inhibition of PI3K but not mitogen-activated protein kinase signaling. Small interfering RNA-mediated knockdown of the p110β catalytic subunit of PI3K results in a decrease in ΔNp63α protein levels in keratinocytes. The results presented herein suggest that regulation of ΔNp63α expression by the PI3K pathway plays a critical role in the survival and proliferative capacity of squamous epithelia.