Iso-coenzyme A

• Published in: The Journal of biological chemistry 2005-04, Vol.280 (17), p.16550-16558
• Main Authors: Burns, Kristi L., Gelbaum, Leslie T., Sullards, M. Cameron, Bostwick, David E., May, Sheldon W.
• Format: Article
• Language: English
• Subjects: Acetyl Coenzyme A - chemistry; beta-Cyclodextrins - chemistry; Chromatography, High Pressure Liquid; Coenzyme A - chemistry; Disulfides - chemistry; Fungal Proteins - chemistry; Magnetic Resonance Spectroscopy; Mass Spectrometry; Models, Chemical; NADP - chemistry; Protein Isoforms; Spectrometry, Mass, Electrospray Ionization; Substrate Specificity; Time Factors
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M411898200

Iso-coenzyme A is an isomer of coenzyme A in which the monophosphate is attached to the 2′-carbon of the ribose ring. Although iso-CoA was first reported in 1959 (Moffatt, J. G., and Khorana, H. G. (1959) J. Am. Chem. Soc. 81, 1265–1265) to be a by-product of the chemical synthesis of CoA, relatively little attention has been focused on iso-CoA or on acyl-iso-CoA compounds in the literature. We now report structural characterizations of iso-CoA, acetyl-iso-CoA, acetoacetyl-iso-CoA, and β-hydroxybutyryl-iso-CoA using mass spectrometry (MS), tandem MS, and homonuclear and heteronuclear NMR analyses. Although the 2′-phosphate isomer of malonyl-CoA was recently identified in commercial samples, previous characterizations of iso-CoA itself have been based on chromatographic analyses, which ultimately rest on comparisons with the degradation products of CoA and NADPH or have been based on assumptions regarding enzyme specificity. We describe a high performance liquid chromatography methodology for separating the isomers of several CoA-containing compounds. We also report here the first examples of iso-CoA-containing compounds acting as substrates in enzymatic acyl transfer reactions. Finally, we describe a simple synthesis of iso-CoA from CoA, which utilizes β-cyclodextrin to produce iso-CoA with high regioselectivity, and we demonstrate a plausible mechanism that accounts for the existence of iso-CoA isomers in commercial preparations of CoA-containing compounds. We anticipate that these results will provide methodology and impetus for investigating iso-CoA compounds as potential pseudo-substrates or inhibitors of the >350 known CoA-utilizing enzymes.