Endothelin-3-converting Enzyme Activity of the KEL1 and KEL6 Phenotypes of the Kell Blood Group System

The Kell blood group protein is a metalloendopeptidase that preferentially cleaves a Trp21-Ile22 bond of big endothelin-3 producing bioactive endothelin-3. Kell is a polymorphic protein, and 25 different phenotypes, because of point mutations resulting in single amino acid substitutions, have been d...

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Bibliographic Details
Published in:The Journal of biological chemistry 2006-03, Vol.281 (11), p.7180-7182
Main Authors: Sha, Quan, Redman, Colvin M., Lee, Soohee
Format: Article
Language:English
Subjects:
Animals
Aspartic Acid Endopeptidases - chemistry
Aspartic Acid Endopeptidases - metabolism
Binding Sites
Catalysis
Cell Line
Chromatography, Affinity
DNA Primers - chemistry
DNA, Complementary - metabolism
Endothelin-Converting Enzymes
Erythrocytes
Humans
Insecta
Isoleucine - chemistry
Kell Blood-Group System
Kinetics
Metalloendopeptidases - chemistry
Metalloendopeptidases - metabolism
Octoxynol - chemistry
Phenotype
Point Mutation
Polymorphism, Genetic
Recombinant Proteins - chemistry
Tryptophan - chemistry
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Summary:The Kell blood group protein is a metalloendopeptidase that preferentially cleaves a Trp21-Ile22 bond of big endothelin-3 producing bioactive endothelin-3. Kell is a polymorphic protein, and 25 different phenotypes, because of point mutations resulting in single amino acid substitutions, have been described. It was recently reported that a recombinant form of KEL1 (K, K1) phenotype, expressed in K562 and HEK293 cells, had no endothelin-3-converting activity, in contrast to the common KEL2 (k, K2) phenotype. We demonstrate that KEL1 red blood cells and also a soluble recombinant form of KEL1 protein (s-Kell KEL1) have similar enzymatic activity as the common Kell phenotype. In addition we show that KEL6 red blood cells, which are more prevalent in persons of African heritage than in Caucasians also have endothelin-3-converting enzyme activity and that the recombinant soluble form of KEL6 protein (s-Kell KEL6) has similar Km values as the wild-type.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M507776200