Aquaporin-2 in the human endolymphatic sac

Objective To detect and localize aquaporin-2 (AQP-2), a water channel regulated by the antidiuretic hormone, in human endolymphatic sac. Material and Methods Human endolymphatic sacs were sampled during removal of vestibular schwannomas via a translabyrinthine approach. Samples were immediately fixe...

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Published in:Acta oto-laryngologica 2004-05, Vol.124 (4), p.449-453
Main Authors: Couloigner, Vincent, Berrebi, Dominique, Teixeira, Marie, Paris, Régine, Florentin, Agnès, Grayeli, Alexis Bozorg, Cluzeaud, Françoise, Sterkers, Olivier, Peuchmaur, Michel, Ferrary, Evelyne
Format: Article
Language:English
Subjects:
antidiuretic hormone
Aquaporin 2
Aquaporins - analysis
Biological and medical sciences
Cytoplasm - chemistry
endolymph
Endolymphatic Sac - chemistry
Epithelium - chemistry
Humans
Immunohistochemistry
In Situ Hybridization
inner ear
Medical sciences
Otorhinolaryngology. Stomatology
Vestibule, Labyrinth - chemistry
water channel
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Summary:Objective To detect and localize aquaporin-2 (AQP-2), a water channel regulated by the antidiuretic hormone, in human endolymphatic sac. Material and Methods Human endolymphatic sacs were sampled during removal of vestibular schwannomas via a translabyrinthine approach. Samples were immediately fixed in 10% formalin (24 h) and embedded in paraffin; in situ hybridization and immunohistochemistry were performed with an AQP-2-specific probe and a polyclonal antibody. Results Both AQP-2 mRNA and protein were detected in the epithelium of the endolymphatic sac. AQP-2 immunostaining was mainly cytoplasmic, suggesting that most AQP-2 was located in intracellular pools. Conclusion In the endolymphatic sac, AQP-2 probably participates in the homeostasis of endolymph; the possibility of reducing the volume of endolymph by inhibiting its expression and membranous insertion using an antidiuretic hormone inhibitor represents a new therapeutic approach for the treatment of Ménière's disease.
ISSN:0001-6489
1651-2251
DOI:10.1080/00016480310000700a