Differentiation of Normal and Neoplastic Cells by Synchronous Fluorescence: Rat Liver Epithelial and Rat Hepatoma Cell Models

In the present study, conventional and synchronous luminescence (SL) were utilized to investigate spectral differences in normal and neoplastic cells. The synchronous fluorescence (SF) method involves scanning simultaneously both emission and excitation wavelengths while keeping a constant wavelengt...

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Bibliographic Details
Published in:Analytical letters 1999-01, Vol.32 (13), p.2583-2594
Main Authors: Watts, Wendi E., Isola, Narayana R., Frazier, Donita, Vo-Dinh, Tuan
Format: Article
Language:English
Subjects:
Biological and medical sciences
cancer diagnosis
hepatoma cell lines
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Miscellaneous. Technology
normal rat liver epithelial
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
synchronous fluorescence
synchronous luminescence
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Summary:In the present study, conventional and synchronous luminescence (SL) were utilized to investigate spectral differences in normal and neoplastic cells. The synchronous fluorescence (SF) method involves scanning simultaneously both emission and excitation wavelengths while keeping a constant wavelength interval between them. This SF procedure simplifies the emission spectrum and provides for greater selectivity and is used to detect subtle differences in the fluorescence emission of the biochemical species of cells from rat tissues. A difference between the fluorescent spectra of the normal rat liver epithelial (RLE) and hepatoma cell lines were detected using synchronous fluorescence. The potential use of SF as a screening tool for cancer diagnosis is discussed.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032719908542989