Determination of Amino Acids in Corn Seed by Gas Chromatography-Mass Spectrometry

A gas chromatography-mass spectrometry method was developed for the determination of free amino acids from corn inbred lines flour. Amino acids were extracted using 6% trichloroacetic acid. The extracts were purified using an ion-exchange solid phase, derivatized as trifluoroacetic butyl esters, and...

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Published in:Analytical letters 2015-01, Vol.48 (1), p.37-46
Main Authors: Culea, Monica, Scrob, Stăncuţa, Suvar, Sonia, Podea, Paula, Haş, Ioan, Muste, Sevastiţa
Format: Article
Language:English
Subjects:
Alanine
Amino acids
Aspartic acid
Corn
Flour
Gas chromatography
GC/MS
Inbred lines
Spectrometry
Spectroscopy
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description A gas chromatography-mass spectrometry method was developed for the determination of free amino acids from corn inbred lines flour. Amino acids were extracted using 6% trichloroacetic acid. The extracts were purified using an ion-exchange solid phase, derivatized as trifluoroacetic butyl esters, and analyzed. The 15 N-methionine was used as the internal standard. The method was repeatable for most of the amino acids (coefficient of variation was in the order of 20%), and good accuracy and limits of detection were obtained. Among the free amino acids determined, the highest concentrations were obtained for aspartic acid, proline, gamma-aminobutyric acid, lysine, alanine, glutamic acid, and histidine. The study established the variation of the free amino acids within the inbred lines.
doi_str_mv 10.1080/00032719.2014.930869
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source Taylor and Francis:Jisc Collections:Taylor and Francis Read and Publish Agreement 2024-2025:Science and Technology Collection (Reading list)
subjects Alanine
Amino acids
Aspartic acid
Corn
Flour
Gas chromatography
GC/MS
Inbred lines
Spectrometry
Spectroscopy
title Determination of Amino Acids in Corn Seed by Gas Chromatography-Mass Spectrometry
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