IDENTIFICATION AND VALIDATION OF DIFFERENTIALLY EXPRESSED GENES IN NEURAL TUBE DEFECTS OF GOLDEN HAMSTER INDUCED BY HYPERTHERMIA USING SUPPRESSION SUBTRACTIVE HYBRIDIZATION

Hyperthermia during early pregnancy can induce neural tube defects (NTD) in embryos. In order to identify the differentially expressed genes that participate in this pathologic course, the authors performed suppression subtractive hybridization in two directions: forward and reverse. Neural tube tis...

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Bibliographic Details
Published in:International journal of neuroscience 2007-08, Vol.117 (8), p.1193-1208
Main Authors: QING, YANG, YINGMAO, GAO, SHAOLING, LI
Format: Article
Language:English
Subjects:
Animals
Cricetinae
Disease Models, Animal
Female
Fever - complications
gene
Gene Expression Profiling - methods
Gene Expression Regulation - physiology
hyperthermia
Male
Mesocricetus
Metabolic Networks and Pathways - physiology
neural tube defects
Neural Tube Defects - etiology
Neural Tube Defects - physiopathology
Nucleic Acid Hybridization - methods
Oligonucleotide Array Sequence Analysis
Pregnancy
Reproducibility of Results
Ribosomal Proteins - metabolism
suppression subtractive hybridization
Transcription Factors - metabolism
Transcription, Genetic - physiology
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Summary:Hyperthermia during early pregnancy can induce neural tube defects (NTD) in embryos. In order to identify the differentially expressed genes that participate in this pathologic course, the authors performed suppression subtractive hybridization in two directions: forward and reverse. Neural tube tissues from golden hamster of normal and hyperthermia groups are used as the samples. As a result, several down-regulated genes were revealed and according to the function of their protein products they were classified into four categories: ribosomal proteins, metabolic enzymes, transcription and translation related factors, and others. On the other hand, the study found that two up-regulated gene fractions were of the same sequence and homology analysis shows that they are homologous to phosphoglycerate kinase 1 (pgk1). Of all these genes, differential expression patterns were confirmed by Northern blot analysis. The study results show that the genes identified have different expression and are stongly related to NTD induced by hyperthermia.
ISSN:0020-7454
1563-5279
1543-5245
DOI:10.1080/00207450600934903