Ultrastructure of freeze-substituted and chemically fixed basidiospores of Gymnosporangium juniperi-virginianae

The results of this study involving basidiospores of G. juniperi-virginianae indicate that freeze-substitution is a valuable technique for TEM studies of fungal spores. Although more spores were damaged in the freeze-substitution protocol than in the chemical fixation protocol, the advantages afford...

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Bibliographic Details
Published in:Mycologia 1988-05, Vol.80 (3), p.356-364
Main Authors: Mims, C.W, Roberson, R.W, Richardson, E.A
Format: Article
Language:English
Subjects:
Basidiospores
Biological and medical sciences
chemical preservation
fixation
Freeze substitution
Frost damage
Fundamental and applied biological sciences. Psychology
Fungal spores
Fungi
Gymnosporangium juniperi-virginianae
Lipids
Mitochondria
Plant cytology, morphology, systematics, chorology and evolution
Spore germination
Spores
Thallophyta
ultrastructure
Vacuoles
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Summary:The results of this study involving basidiospores of G. juniperi-virginianae indicate that freeze-substitution is a valuable technique for TEM studies of fungal spores. Although more spores were damaged in the freeze-substitution protocol than in the chemical fixation protocol, the advantages afforded by the former technique greatly outweighed losses caused by such damage. The quality of fixation provided by freeze-substitution was equal to or better than that provided by chemical fixation for virtually every spore component except lipid droplets. Freeze-substitution also yielded information that was not available from chemically fixed samples. Such information related to Golgi bodies and vesicles, multivesicular bodies, vacuoles and microtubules.
ISSN:0027-5514
1557-2536
DOI:10.1080/00275514.1988.12025550