Observations of Gonapodya in Pure Culture: Growth, Development and Cell Wall Characterization

Gonapodya prolifera was isolated from a pond in Athens, Georgia, and its development in pure culture is reported for the first time. While the fungus grew well on a diversity of media, the morphology was closest to published descriptions when the fungus was grown on dogwood fruits with twigs held un...

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Bibliographic Details
Published in:Mycologia 1993-01, Vol.85 (1), p.38-45
Main Authors: Fuller, Melvin S., Clay, Ronald P.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Biology and Ultrastructure
Cell walls
cellulose
Chitin
Freshwater
Fruits
Fundamental and applied biological sciences. Psychology
Fungi
Glycogen
gold labeling
Gonapodya
Hyphae
Music analysis
Plant cytology, morphology, systematics, chorology and evolution
pseudosepta
pure culture
Sporangia
Starches
Thallophyta
Zoospores
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Summary:Gonapodya prolifera was isolated from a pond in Athens, Georgia, and its development in pure culture is reported for the first time. While the fungus grew well on a diversity of media, the morphology was closest to published descriptions when the fungus was grown on dogwood fruits with twigs held under reduced oxygen conditions. The distributions of chitin, cellulose, glycogen and N-acetyl-D-galactosamine residues in the cytoplasm, cell walls and pseudosepta of freeze substituted hyphae were studied with gold-complexed enzyme and lectin probes. The presence of cellulose in the cell walls of this member of the Chytridiomycetes was substantiated by compositional and methylation analyses of a cell wall extraction that was selective for cellulose. The observation that Helix pomatia agglutinin binds to glycogen in thin section is discussed, as is the first reported detection of cellulose in a member of the Chytridiomycetes.
ISSN:0027-5514
1557-2536
DOI:10.1080/00275514.1993.12026243