Flow cytometric expression of CD71, CD81, CD44 and CD39 in B cell lymphoma

Flow cytometry is a useful ancillary tool for the diagnosis of nodal B cell lymphomas. Well-established antigens have diagnostic limitations. This study aimed to assess the expression of CD71, CD81, CD44 and CD39 by flow cytometry in B cell lymphomas. Expression of these 4 antigens was queried in 18...

Full description

Saved in:
Bibliographic Details
Published in:Scandinavian journal of clinical and laboratory investigation 2021-09, Vol.81 (5), p.413-417
Main Authors: Espasa, Andrea, Tapia, Gustavo, Vergara, Sara, Raya, Minerva, Juncà, Jordi, Sorigue, Marc
Format: Article
Language:English
Subjects:
Antigens, CD - analysis
Apyrase - analysis
CD39
CD44
CD71
CD81
Female
flow cytometry
Flow Cytometry - methods
Humans
Hyaluronan Receptors - analysis
Lymphoma
Lymphoma, B-Cell - immunology
Male
Receptors, Transferrin - analysis
Tetraspanin 28 - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Flow cytometry is a useful ancillary tool for the diagnosis of nodal B cell lymphomas. Well-established antigens have diagnostic limitations. This study aimed to assess the expression of CD71, CD81, CD44 and CD39 by flow cytometry in B cell lymphomas. Expression of these 4 antigens was queried in 185 samples with a diagnosis of a B cell lymphoma according to a histological examination of the lymph node and the World Health Organization (WHO) classification (follicular lymphoma [FL, n = 96], diffuse large B cell lymphoma/High grade B cell lymphoma [DLBCL/HGBH, n = 48], marginal zone lymphoma/lymphoplasmacytic lymphoma [MZL/LPL, n = 14], chronic lymphocytic leukemia/small lymphocytic lymphoma [CLL, n = 10], mantle cell lymphoma [MCL, n = 11], Burkitt lymphoma [BL, n = 4] and other [n = 2]). CD81 was bright and CD44 was dim in germinal center-derived malignancies, particularly aggressive lymphomas (BL and CD10-positive DLBCL/HGBL). CD81 was very dim in CLL. CD71 was bright in aggressive lymphomas (DLBCL/HGBL and BL). CD39 was bright in CD10-negative DLBCL. CD71 appeared valuable in the differential diagnosis between indolent and aggressive lymphomas, CD39 between CD10-negative DLBCL and MZL/LPL and CD81 between MCL and CLL. To conclude, we report the expression of CD71, CD81, CD44 and CD39 by FC in B cell lymphomas. Further studies will have to determine the value they add to specific FC panels.
ISSN:0036-5513
1502-7686
DOI:10.1080/00365513.2021.1929446