Analysis of p53 Gene Mutations in Helicobacter pylori-Associated Gastritis Mucosa in Endoscopic Biopsy Specimens

Background: No previous report has shown the relationship between Helicobacter pylori infection and a direct sequence analysis of p53 gene mutation in a biopsy sample of human gastric mucosa. Methods: A total of 60 endoscopic biopsies samples (21 patients with H. pylori-positive gastritis and 9 pati...

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Bibliographic Details
Published in:Scandinavian journal of gastroenterology 1999, Vol.34 (5), p.474-477
Main Authors: MURAKAMI, K, FUJIOKA, T, OKIMOTO, T, MITSUISHI, Y, ODA, T, NISHIZONO, A, NASU, M
Format: Article
Language:English
Subjects:
Adult
Aged
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Base Sequence
Biological and medical sciences
Female
Gastric Mucosa - pathology
Gastritis - microbiology
Gastritis - pathology
Genes, p53 - genetics
Helicobacter Infections - genetics
Helicobacter Infections - pathology
Helicobacter pylori
Human bacterial diseases
Humans
Infectious diseases
Male
Medical sciences
Middle Aged
Mutation
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Summary:Background: No previous report has shown the relationship between Helicobacter pylori infection and a direct sequence analysis of p53 gene mutation in a biopsy sample of human gastric mucosa. Methods: A total of 60 endoscopic biopsies samples (21 patients with H. pylori-positive gastritis and 9 patients with H. pylori-negative gastritis), including antral mucosa and corpus mucosa, were used in this study. Direct DNA sequencing of exons 5, 6, 7, and 8 of the p53 gene was performed by the dyedeoxy terminator method. Results: Mutations in the p53 gene were identified in non-hot spot codons in exon 7 and 8 in 11 of 21 samples (52.4%) from H. pylori-positive gastritis patients. There was no mutation in H. pylori-negative gastritis patients. Conclusions: This finding shows that H. pylori infection can induce p53 point mutations and appears to be involved in the pathway leading to dysplasia or carcinoma.
ISSN:0036-5521
1502-7708
DOI:10.1080/003655299750026191