Pathogenicity of Heterorhabditis nematodes isolated from north-western Himalaya against the larvae of Plutella xylostella (L.) (Lepidoptera: Plutellidae)

• Published in: Annales de la Société entomologique de France 2017-05, Vol.53 (3), p.204-210
• Main Authors: Vashisth, Sumit, Chandel, Y.S., Chandel, R.S., Sharma, P.K.
• Format: Article
• Language: English
• Subjects: biological control; cole crops; diamondback moth; entomopathogenic nematodes
• ISSN: 0037-9271; 2168-6351
• DOI: 10.1080/00379271.2017.1324320

The efficacy of three entomopathogenic nematodes (Heterorhabditis spp.), from north western Himalaya, India was studied against the diamondback moth, Plutella xylostella (Linnaeus, 1758) (Lepidoptera: Plutellidae), under laboratory conditions. The larvae were exposed to 10, 20, 30 and 40 infective juveniles (IJs) of each nematode species for different time periods and they were found to be susceptible to all the EPNs tested. However, the susceptibility of larvae to nematode infection varied according to the dosages of IJs and their exposure periods. The efficacy of these indigenous entomopathogenic nematodes was also evaluated against the commercially available entomopathogenic nematode H. indica. An indigenous isolate, H. bacteriophora (HRJ), along with the commercial isolate H. indica recorded 100.0% mortality of insect larvae in 96 h exposure time against third instar larvae of P. xylostella. However, it was noticed that with the advancement of larval stage its mortality rate reduces and vice versa with the exposure period. All the tested nematode species were also found to reproduce within the host and produced infective juveniles. In conclusion, the evidence obtained in this study suggests that all the three indigenous EPN species are virulent enough to produce 100% mortality of larvae of P. xylostella. These EPN species thus have potential for the management of P. xylostella under integrated management practices.