MicroRNA-655-3p regulates Echinacea purpurea mediated activation of ABCG2

1. The aim of this study was to investigate the regulatory effect of Echinacea purpurea (EP) on efflux transporters ABCB1 and ABCG2 and to identify specific microRNAs contributing to their post-transcriptional regulation. 2. ABCB1 and ABCG2 levels were assessed in human hepatoblastoma HepG2 cells tr...

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Bibliographic Details
Published in:Xenobiotica 2018-10, Vol.48 (10), p.1050-1058
Main Authors: Awortwe, Charles, Kaehler, Meike, Rosenkranz, Bernd, Cascorbi, Ingolf, Bruckmueller, Henrike
Format: Article
Language:English
Subjects:
ABC transporter
CAR
drug interaction
Echinacea
gene regulation
microRNA
PXR
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Summary:1. The aim of this study was to investigate the regulatory effect of Echinacea purpurea (EP) on efflux transporters ABCB1 and ABCG2 and to identify specific microRNAs contributing to their post-transcriptional regulation. 2. ABCB1 and ABCG2 levels were assessed in human hepatoblastoma HepG2 cells treated with 50 µg/mL methanolic extract of commercial EP capsules for different durations. The microRNA expression profile of HepG2 cells after EP treatment was evaluated and in silico target prediction was subsequently conducted to identify specific microRNAs with binding sites in the 3′-UTR of ABCB1 and ABCG2. Luciferase reporter gene assays and site-directed mutagenesis were used to confirm the binding site of identified microRNA within the 3′-UTR of the target gene. 3. EP increased ABCB1 (10-fold ± 3.4, p 
ISSN:0049-8254
1366-5928
DOI:10.1080/00498254.2017.1390624