Latex particles sensitized with proteins of Leptospira interrogans for application in immunoagglutination assays

Physical adsorption and chemical coupling of recombinant proteins of Leptospira interrogans onto polystyrene and core-shell carboxylated particles were, respectively, investigated with the aim of producing latex-protein complexes to be used in immunoagglutination assays able to detect the leptospiro...

Full description

Saved in:
Bibliographic Details
Published in:International journal of polymeric materials 2017-10, Vol.66 (15), p.791-797
Main Authors: Prochetto, Santiago D., Gonzalez, Verónica D. G., Garcia, Valeria S., Vanasco, Norma B., Lottersberger, Javier, Gugliotta, Luis M.
Format: Article
Language:English
Subjects:
Adsorption
Antigens
Assaying
Bacterial infections
Carboxyl group
Coordination compounds
Coupling
Covalence
Immunoagglutination
Immunoassay
Latex
latex-protein complex
leptospirosis
Polystyrene resins
Proteins
Surface chemistry
Turbidimetry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Physical adsorption and chemical coupling of recombinant proteins of Leptospira interrogans onto polystyrene and core-shell carboxylated particles were, respectively, investigated with the aim of producing latex-protein complexes to be used in immunoagglutination assays able to detect the leptospirosis disease in either humans or animals. To this effect, a protein lysate of crude extracts was evaluated, and sensitizations were carried out at different pHs, with the antigenic proteins approach to the particle surface favored at pH close to their isoelectric point. In the covalent coupling experiments, high fractions of proteins were chemically bound to carboxyl groups on the particle surface and higher densities of linked proteins were obtained for particles exhibiting greater carboxyl group densities. The produced latex-protein complexes were tested in immunoagglutination assays, by turbidimetry and a visual method, from a panel that included positive and negative bovine, canine, and human sera. The area under receiver operating characteristic curves was used as an index of accuracy. The complexes obtained by covalent coupling of proteins on the latex of higher density of carboxyl groups allowed an acceptable discrimination between the studied positive and negative sera.
ISSN:0091-4037
1563-535X
DOI:10.1080/00914037.2016.1276058