In vitro cytogenetic evaluation of the particular combination of flurbiprofen and roxithromycin

• Published in: Drug and chemical toxicology (New York, N.Y. 1978) N.Y. 1978), 2017-07, Vol.40 (3), p.326-332
• Main Authors: Timocin, Taygun, Husunet, Mehmet Tahir, Valipour, Ebrahim, Norizadeh Tazehkand, Mostafa, Celik, Rima, Topaktas, Mehmet, Ila, Hasan B.
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - toxicity; Anti-Inflammatory Agents, Non-Steroidal - administration & dosage; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Anti-Inflammatory Agents, Non-Steroidal - toxicity; Cell Division - drug effects; Cells, Cultured; cytotoxicity; DNA Damage; Dose-Response Relationship, Drug; Drug Interactions; Female; Flurbiprofen; Flurbiprofen - administration & dosage; Flurbiprofen - pharmacology; Flurbiprofen - toxicity; genotoxicity; Humans; Lymphocytes - drug effects; Lymphocytes - pathology; Male; Micronuclei, Chromosome-Defective - chemically induced; micronucleus; oxidative stress; Oxidative Stress - drug effects; Plasmids; Roxithromycin; Roxithromycin - administration & dosage; Roxithromycin - pharmacology; Roxithromycin - toxicity; Young Adult
• ISSN: 0148-0545; 1525-6014
• DOI: 10.1080/01480545.2016.1223097

Flurbiprofen (FLB) (anti-inflammatory and analgesic drug) and roxithromycin (RXM) (antibiotic) were widely used in world wide. This study deals with investigation of genotoxicity, cytotoxicity, and oxidative stress effects of a particular combination of these drugs in human cultured lymphocytes. Also, DNA damaging-protective effects of combination of these drugs were analyzed on plasmid DNA. Human lymphocytes were treated with different concentrations (FLB + RXM; 10 μg/mL + 25 μg/mL, 15 μg/mL + 50 μg/mL, and 20 μg/mL + 100 μg/mL) of the drugs following by study of their genotoxic and cytotoxic effects by analysis of cytokinesis-block micronucleus test and nuclear division index, respectively. The effect of the combination in aspect of anti-oxidative and DNA damaging activity was evaluated on Pet-22b plasmid. According to our results, the combination of FLB and RXM did not show a notable genotoxic effect on cells. Although each of the substances had been shown as a cytotoxic agent by previous researchers, in this research, the combination of these drugs did not exhibit any adverse effect on cell division. FLB had DNA protection effect against H 2 O 2 while in combination with RXM had not the same effect on the plasmid.