Protective effects of Bidens pilosa on hepatoxicity and nephrotoxicity induced by carbon tetrachloride in rats

• Published in: Drug and chemical toxicology (New York, N.Y. 1978) N.Y. 1978), 2021-01, Vol.44 (1), p.64-74
• Main Authors: Pegoraro, Cristiane Martinez Ruiz, Nai, Gisele Alborghetti, Garcia, Leonardo Alves, Serra, Fernanda de Maria, Alves, Juliana Apolônio, Chagas, Pedro Henrique Nahas, Oliveira, Décio Gomes de, Zocoler, Marcos Alberto
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Antimutagenic Agents - pharmacology; Bidens; Bidens pilosa; Carbon Tetrachloride; Chemical and Drug Induced Liver Injury - etiology; Chemical and Drug Induced Liver Injury - metabolism; Chemical and Drug Induced Liver Injury - pathology; Chemical and Drug Induced Liver Injury - prevention & control; Comet Assay; cyclophosphamide; Disease Models, Animal; DNA Damage; Drugs, Chinese Herbal - pharmacology; genotoxicity; Kidney - drug effects; Kidney - metabolism; Kidney - pathology; Kidney Diseases - chemically induced; Kidney Diseases - metabolism; Kidney Diseases - pathology; Kidney Diseases - prevention & control; liver; Liver - drug effects; Liver - metabolism; Liver - pathology; Male; Micronuclei, Chromosome-Defective - chemically induced; Micronuclei, Chromosome-Defective - drug effects; Micronucleus Tests; Rats; Rats, Wistar
• ISSN: 0148-0545; 1525-6014
• DOI: 10.1080/01480545.2018.1526182

The aim of this study was to assess the protective effects of oral and topical treatment with Bidens pilosa (BP) against carbon tetrachloride (CCl 4 ) − induced toxicity. Fifty-six rats were divided into seven groups: A: CCl 4 only; B: CCl 4 +oral BP; C: CCl 4 and topical BP; D: CCl 4 +oral and topical BP; E: oral BP only; F: negative control; and G: positive control (cyclophosphamide). The animals were treated for 10 weeks. Blood samples were collected for tests of hepatic and renal function, and fragments of the liver, spleen, pancreas, kidney, and intestine were collected for histopathological analyses. Cells from the femoral bone marrow were used for a micronucleus test and 'comet assay'. Statistically significant differences were observed in the levels of gamma-glutamyl transpeptidase (GGT), albumin, urea and creatinine, hepatic inflammation, renal tubular lesion, and inflammation of the intestinal mucosa between the BP-treated groups and untreated group. The median number of micronuclei in group A was 4.00, in group G was 9.00 and in the other groups was 0.00. Group A had the lowest number of cells with a score of 0 and the greatest number with scores of 3 and 4, similar to the results obtained from group G using the 'comet assay'. Thus, BP effectively protected against the toxic effects of CCl 4 on the liver, kidney, and intestine and exerted an antimutagenic effect on rats exposed to CCl 4 .