Lobularia maritima leave extract, a nutraceutical agent with antioxidant activity, protects against CCl 4 -induced liver injury in mice

• Published in: Drug and chemical toxicology (New York, N.Y. 1978) N.Y. 1978), 2022-03, Vol.45 (2), p.604-616
• Main Authors: Ben Hsouna, Anis, Dhibi, Sabah, Dhifi, Wissal, Ben Saad, Rania, Brini, Faical, Hfaidh, Najla, Almeida, Jackson Roberto Guedes da Silva, Mnif, Wissem
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - pharmacology; Brassicaceae; Carbon Tetrachloride - toxicity; Chemical and Drug Induced Liver Injury - drug therapy; Chemical and Drug Induced Liver Injury - metabolism; Chemical and Drug Induced Liver Injury - prevention & control; Chemical and Drug Induced Liver Injury, Chronic - metabolism; Dietary Supplements; Lipid Peroxidation; Liver; Mice; Oxidative Stress; Plant Extracts - chemistry
• ISSN: 0148-0545; 1525-6014
• DOI: 10.1080/01480545.2020.1742730

( , Brassicaceae), commonly known as sweet alyssum, is an annual ornamental halophyte widely spread along the Tunisian seashore. leaf ethanol extract was tested in an experimental model of hepatotoxicity induced by carbon tetrachloride (CCl ). extract was found to possess antioxidant activity by scavenging the DPPH radical (IC = 45 μg/mL), reducing/chelating iron ions and inhibiting liver lipid peroxidation induced by FeSO . The levels of total phenolics and flavonoids were 175 ± 2.66 mg GAE/g, and 35 ± 2.88 mg QE/g respectively. Moreover, HPLC analysis revealed six compounds, namely gallic, salicylic, ellagic and ferulic acids as well as catechin and quercetin. A mice model of acute liver injury was successfully established after a single intraperitoneal injection of CCl , as evidenced by histological analysis, Masson trichrome and Sirius red staining. Compared with the CCl intoxicated group, the treatment resulted to reduce the liver serum marker enzymes, lipid peroxidation and increased the activities of antioxidant enzymes with further amelioration in the oxidative stress. The present findings discover the therapeutic potentials of empowered with promising natural leads for the treatment of oxidative stress associated health disorders by attenuating free radicals, inhibiting lipid peroxidation, and upregulating the tissue-specific antioxidant enzymes.