Isolation and Identification of Extremophilic Bacteria with Potential as Plant Growth Promoters (Pgpb) of A Geothermal Site: A Case Study

'Los Negritos" is an extremophile geothermal site from Michoacan State, Mexico. Evidence of the diversity of its microbiota and its potential is still lacking. This study aimed to evaluate the bacterial communities by 16S metagenomics of the site and the potential of culturable bacteria is...

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Bibliographic Details
Published in:Geomicrobiology journal 2021-05, Vol.38 (5), p.436-450
Main Authors: Tuesta-Popolizio, Diego A., Velázquez-Fernández, Jesús Bernardino, Rodriguez-Campos, Jacobo, Contreras-Ramos, Silvia Maribel
Format: Article
Language:English
Subjects:
Acetic acid
Ammonium
Ammonium compounds
Bacteria
Extremophiles
halotolerant
Indoleacetic acid
Metagenomics
Microbiological strains
Microbiota
phosphorous solubilization
Plant growth
Plant growth promoters
Plant growth substances
plant growth-promoting rhizobacteria
Plant hormones
saline-sulfur
Solubilization
Tryptophan
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Summary:'Los Negritos" is an extremophile geothermal site from Michoacan State, Mexico. Evidence of the diversity of its microbiota and its potential is still lacking. This study aimed to evaluate the bacterial communities by 16S metagenomics of the site and the potential of culturable bacteria isolated from the site by promoting growth plants (PGP) traits. Gemmatimonadetes phylum was the most abundant, followed by Actinobacteria, Alphaproteobacteria, and Firmicutes. A total of 15 culturable strains were isolated and identified. They belong to five different species: Bacillus seohaeanensis, Jeotgalibacillus alkaliphilus, Halobacillus andaensis, Thalassobacillus devorans, and Halobacillus campisalis. The phytohormone indole-3-acetic acid (IAA) production was in the interval from 14.78 to 46.52 mg L −1 . The best IAA producers were T. devorans E18 and H. campisalis E20 with tryptophan and H. andaensis, T. devorans, and H. campisalis without tryptophan ranging 39.8 to 51.5 mg L −1 . Phosphorous solubilization capability was detected in J. alkaliphilus and T. devorans, whereas B. seohaeanensis was the highest ammonium producer (34.1 ± 0.52 mg L −1 ) and H. andaensis was positive for siderophore production. These results demonstrate that, despite the low diversity, bacteria isolated from 'Los Negritos' have the potential for plant growth promotion.
ISSN:0149-0451
1521-0529
DOI:10.1080/01490451.2021.1879972