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Isolation of Trypsin from Bovine Pancreas Using Immobilized Benzamidine and Peptide CTPR Ligands in Expanded Beds
Peptide CTPR and p-amino benzamidine (PAB) immobilized on Streamline™ were utilized as the chromatographic matrices for trypsin purification from bovine pancreas. By using a clarified pancreas extract, maximum capacity for CTPR-Streamline was 47.4 mg/mL and for PAB-Streamline 78.9 mg/mL while Kd val...
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Published in: | Separation science and technology 2005-12, Vol.40 (16), p.3277-3287 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Peptide CTPR and p-amino benzamidine (PAB) immobilized on Streamline™ were utilized as the chromatographic matrices for trypsin purification from bovine pancreas. By using a clarified pancreas extract, maximum capacity for CTPR-Streamline was 47.4 mg/mL and for PAB-Streamline 78.9 mg/mL while Kd values were 0.39 and 0.38 respectively. Dynamic capacity was 23.0 and 46.0 mg/mL for CTPR- and PAB-Streamline respectively. When the purification process was applied to unclarified pancreas extract in the expanded-bed adsorption mode, 80% trypsin recovery with a purification factor of 18.7 was achieved. Cationic and anionic trypsin obtained from the affinity column were separated by ion-exchange chromatography. |
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ISSN: | 0149-6395 1520-5754 |
DOI: | 10.1080/01496390500423631 |