Optimized Extraction and High Selective Purification of Flavonoids and Intermediate Polar Nitrogen-Containing Constituents from Brassica campestris L. Pollen

For the first time, an efficient purification process of flavonoids and intermediate polar nitrogen-containing constituents from Brassica campestris L. pollen with the help of an improved polystyrene based matrix, LS305 was developed. Three methods (Ultra Fast Liquid Chromatographic with Ultraviolet...

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Bibliographic Details
Published in:Separation science and technology 2013-10, Vol.48 (15), p.2352-2362
Main Authors: Fan, Siyang, Lu, Guoqing, Yang, Yifang, Xiao, Wei, Wang, Zhenzhong, Ding, Gang, Zhu, Huarong, Yuan, Shaowei
Format: Article
Language:English
Subjects:
Arrays
Brassica
Brassica campestris L. Pollen
Constituents
Diodes
Flavonoids
Liquids
nitrogen-containing constituents
Pollen
Purification
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Summary:For the first time, an efficient purification process of flavonoids and intermediate polar nitrogen-containing constituents from Brassica campestris L. pollen with the help of an improved polystyrene based matrix, LS305 was developed. Three methods (Ultra Fast Liquid Chromatographic with Ultraviolet/Diode Array Detector, spectrophotometric, and automatic Kjeldahl) have been applied for the determination and quantification of flavonoids and nitrogen. With the help of the purification process, the flavonoids content was increased from ∼16% to ∼45% (w/w). ∼6% (w/w) nitrogen originated from intermediate polar nitrogen-containing compounds was retained. These results would contribute to improve industrial processes.
ISSN:0149-6395
1520-5754
DOI:10.1080/01496395.2013.804089