Isolation and Purification of Isoquercitrin and Quercitrin from sf Hypericum Japonicum Thunb.ex Murray by Counter-Current Chromatography

Isoquercitrin and quercitrin were successfully isolated and purified from Hypericum japonicum Thunb.ex Murray by counter-current chromatography with a solvent system of n-hexane-ethyl acetate-methanol-water (1:7:1:7, v/v/v/v) in one step. From 100 mg of the extract of Hypericum japonicum Thunb.ex Mu...

Full description

Saved in:
Bibliographic Details
Published in:Separation science and technology 2014-03, Vol.49 (5), p.778-782
Main Authors: Xie, Zhisheng, Huang, Jieyun, Xie, Zhiyong, Yu, Xiaoxue, Yang, Mei, Yang, Depo, Xu, Xinjun
Format: Article
Language:English
Subjects:
CCC
Chromatography
hypericum japonicum
isoquercitrin
Nuclear magnetic resonance
Purification
Purity
quercitrin
Separation
Solvents
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Isoquercitrin and quercitrin were successfully isolated and purified from Hypericum japonicum Thunb.ex Murray by counter-current chromatography with a solvent system of n-hexane-ethyl acetate-methanol-water (1:7:1:7, v/v/v/v) in one step. From 100 mg of the extract of Hypericum japonicum Thunb.ex Murray, 9.8 mg of isoquercitrin and 12 mg of quercitrin were obtained with the purities of 95.9% and 99.1%, respectively, as determined by HPLC. Their structures were identified by UV, MS, and NMR analysis. In this study, a rapid method for isolation and purification of the two major compounds from Hypericum japonicum Thunb.ex Murray crude extract was established.
ISSN:0149-6395
1520-5754
DOI:10.1080/01496395.2013.864310