Single step recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction

Recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction was studied with maximal possible crude enzyme loads. In polyethylene glycol/dextran and polyethylene glycol/salt systems the influences of molecular weight and concentration of polyethylene glycol, phase-forming salt and...

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Bibliographic Details
Published in:Separation science and technology 2016-03, Vol.51 (4), p.622-628
Main Authors: Antov, Mirjana G., Ivetić, Darjana Ž., Knežević Jugović, Zorica D.
Format: Article
Language:English
Subjects:
Aqueous two-phase extraction
bioseparation
Enzymes
Extraction
Lipase
Molecular weight
partitioning
Penicillium
Polyethylene glycol
Purification
Recovery
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Summary:Recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction was studied with maximal possible crude enzyme loads. In polyethylene glycol/dextran and polyethylene glycol/salt systems the influences of molecular weight and concentration of polyethylene glycol, phase-forming salt and phase volume ratio were evaluated. Lipase partition coefficient 9 followed by the top phase yield 95.7% and purification factor 3.4 were achieved in 15% (w w −1 ) polyethylene glycol 4000/15% (w w −1 ) KH 2 PO 4 /70% (w w −1 ) crude enzyme. Efficient single-step recovery of lipase followed by partial enzyme purification indicated possible integration of production and primary bioseparation step by aqueous two-phase extraction. By varying phase volume ratio, the concentration of phosphate was reduced without decrease in lipase recovery.
ISSN:0149-6395
1520-5754
DOI:10.1080/01496395.2015.1117106