Preparation of reference standard extract of Lonicera hypoglauca Miq. with macroporous resin following a scale-up separation

Bench-scale and pilot-scale methods were developed for the separation of extracted caffeoylquinic acids (CQAs) also called reference standard extracts (RSE) from defined source Lonicerae species using macroporous resin. Various separation parameters of the process were optimized such as Lonicera hyp...

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Bibliographic Details
Published in:Separation science and technology 2021-05, Vol.56 (8), p.1342-1350
Main Authors: Zhong, Jia-Lun, Muhammad, Nadeem, Bian, Ping-Feng, Shan, Ying-Ying, Yan, Wei-Dong
Format: Article
Language:English
Subjects:
Caffeoylquinic acid
Ethanol
Flow rates
Flow velocity
HPLC
Lonicera
Lonicera hypoglauca
macroporous resin
Process parameters
reference standard extract
Resins
Separation
Solvent extraction
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Summary:Bench-scale and pilot-scale methods were developed for the separation of extracted caffeoylquinic acids (CQAs) also called reference standard extracts (RSE) from defined source Lonicerae species using macroporous resin. Various separation parameters of the process were optimized such as Lonicera hypoglauca Miq. batch No. 4, 75% ethanol as extraction solvent, SD3000 macroporous resin, 3 mg/mL of total CQA loading solutions, 2 BV/h of adsorption and desorption flow rates and ethanol-water (30:70, 80:20, v/v) as eluent in gradient mode. The pilot-scale experiments were evaluated in six cycles. The quantitative analysis of CQA using RSE and mixed external standards were compared.
ISSN:0149-6395
1520-5754
DOI:10.1080/01496395.2020.1778726