Egg white protein polymer: an affinity matrix for protease enrichment and isolation

Egg white was precipitated with acetone and cross-linked with glutaraldehyde. Protease specificity of the polymer was investigated with different types of proteases and non-protease proteins. Protein polymer effectively bound trypsin, papain and the different proteases from pancreatin. SDS-PAGE resu...

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Bibliographic Details
Published in:Separation science and technology 2022-01, Vol.57 (1), p.103-111
Main Authors: Menfaatli, Esra, Zihnioğlu, Figen
Format: Article
Language:English
Subjects:
Acetone
affinity enrichment
affinity purification
Albumen
biopolymers
Diffusion plating
egg white protein
Eggs
Enrichment
Gel electrophoresis
Pancreatin
Papain
Polymers
Protease
Protease enrichment
protease removal
Proteinase
Proteins
Sodium lauryl sulfate
Specificity
Trypsin
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Summary:Egg white was precipitated with acetone and cross-linked with glutaraldehyde. Protease specificity of the polymer was investigated with different types of proteases and non-protease proteins. Protein polymer effectively bound trypsin, papain and the different proteases from pancreatin. SDS-PAGE results and diffusion agar plate tests supported the protease-binding efficiency of the polymer. In conclusion, egg white protein polymer can specifically bind proteases and it is a convenient and cost-effective material for enrichment, isolation, and removal of proteases.
ISSN:0149-6395
1520-5754
DOI:10.1080/01496395.2021.1887235