Relationship among colonocyte proliferation, differentiation, and apoptosis as a function of diet and carcinogen

To determine the relationship among colonocyte proliferation, differentiation, and apoptosis as a function of fiber and carcinogen, we conducted a 2 X 2 factorial design study with two fibers (pectin or cellulose) and two injection protocols (azoxymethane or saline) in male Sprague-Dawley rats. Rats...

Full description

Saved in:
Bibliographic Details
Published in:Nutrition and cancer 1997, Vol.28 (1), p.20-29
Main Authors: Hong, M.Y, Chang, W.C.L, Chapkin, R.S, Lupton, J.R
Format: Article
Language:English
Subjects:
ANIMAL MODELS
Animals
APOPTOSE
APOPTOSIS
AZOXYMETHANE
Azoxymethane - pharmacology
Biological and medical sciences
Bromodeoxyuridine - metabolism
Carcinogenesis, carcinogens and anticarcinogens
Carcinogens - pharmacology
CELL DIFFERENTIATION
Cell Differentiation - drug effects
CELL DIVISION
Cell Division - drug effects
CELLS
CELLULE
CELLULOSE
CELULAS
CELULOSA
Colon - cytology
COMPOSE ORGANOAZOTE
COMPUESTO ORGANICO DEL NITROGENO
DIET
DIETA
Dietary Fiber - administration & dosage
DIFERENCIACION CELULAR
DIFFERENCIATION CELLULAIRE
DISTAL COLON
DIVISION CELLULAIRE
DIVISION CELULAR
DNA - biosynthesis
Eating
Epithelial Cells
EXPERIMENTACION
EXPERIMENTATION
Feces
Foods and miscellaneous
INTESTIN
Intestinal Mucosa - cytology
INTESTINES
INTESTINOS
Male
Medical sciences
MEMBRANA MUCOSA
MODELE ANIMAL
MODELOS ANIMALES
MORPHOLOGY
MUCOUS MEMBRANE
MUQUEUSE
ORGANIC NITROGEN COMPOUNDS
PECTINAS
PECTINE
PECTINS
Pectins - pharmacology
PROXIMAL COLON
RAT
RATA
RATS
Rats, Sprague-Dawley
REGIME ALIMENTAIRE
Tumors
Weight Gain
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To determine the relationship among colonocyte proliferation, differentiation, and apoptosis as a function of fiber and carcinogen, we conducted a 2 X 2 factorial design study with two fibers (pectin or cellulose) and two injection protocols (azoxymethane or saline) in male Sprague-Dawley rats. Rats were killed six weeks after the injections, and in vivo cell proliferation was measured by incorporation of bromodeoxyuridine into DNA, differentiation by binding of the lectin Dolichos biflorus agglutinin, and apoptosis by immunoperoxidase detection of digoxigenin-labeled genomic DNA. In the proximal colon, pectin decreased differentiation and apoptosis, resulting in a greater number of cells per crypt column. In the distal colon, pectin increased cell proliferation, resulting in more crypts per millimeter of colon and a greater number of surface cells. Azoxymethane increased cell proliferation and decreased differentiation and apoptosis in the proximal and the distal colon. This resulted in a greater number of surface cells proximally and more crypts per millimeter of colon distally. These results illustrate the importance of considering all three parameters (proliferation, differentiation, and apoptosis) when evaluating neoplastic growth
ISSN:0163-5581
1532-7914
DOI:10.1080/01635589709514548