Ultrastructural changes induced by açaí (Euterpe oleracea Mart) in MCF-7 breast cancer cell line

Açaí (Euterpe oleracea Mart) is an Amazon plant with many biological properties. Previous report of this group evidenced autophagy induction after treatment with açaí seed extract in MCF-7 breast cancer cell lines by acridine orange assay. The aim of this study was to evaluate the ultrastructural ch...

Full description

Saved in:
Bibliographic Details
Published in:Ultrastructural pathology 2022-11, Vol.46 (6), p.511-518
Main Authors: da Silva, Marcos Antonio Custódio Neto, Soares, Camila Simões, Borges, Kátia Regina Assunção, Wolff, Laís Araujo Souza, Barbosa, Maria Do Carmo Lacerda, Nascimento, Maria Do Desterro Soares Brandão, Carvalho, João Ernesto de
Format: Article
Language:English
Subjects:
Acridine Orange
Antioxidants - pharmacology
autophagy
açaí
Breast Neoplasms
Euterpe - chemistry
Female
Humans
MCF-7
MCF-7 Cells
Plant Extracts - pharmacology
Ultrastructural changes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Açaí (Euterpe oleracea Mart) is an Amazon plant with many biological properties. Previous report of this group evidenced autophagy induction after treatment with açaí seed extract in MCF-7 breast cancer cell lines by acridine orange assay. The aim of this study was to evaluate the ultrastructural changes induced by açaí seed extract in MCF-7 breast cancer cell lines. First, MCF- 7 breast cancer cell line viability was evaluated by MTT assay. Acridine orange assay showed increase in the acidic compartments, suggesting autophagolysosome formation. These cells were treated with 25 μg/ml for 24 h and evaluated by transmission electron microscopy (MET). This analysis showed that açaí seed extract induced autophagy, confirmed by autophagolysosome formation. Furthermore, açaí seed extract increased the number of mitochondria, suggesting the enrollment of reactive oxygen species in autophagy.
ISSN:0191-3123
1521-0758
DOI:10.1080/01913123.2022.2141404