A Fast Histochemical Staining Method to Identify Hyaline Droplets in the Rat Kidney

Hyaline droplet formation in the proximal tubular cells of the kidney commonly occurs under different pathological conditions in experimental animals. In rats, intracytoplasmic hyaline droplet formation is predominantly associated with accumulation of the male rat-specific α2u-globulin, whereas unde...

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Bibliographic Details
Published in:Toxicologic pathology 2003-07, Vol.31 (4), p.462-464
Main Authors: De Rijk, Eveline P.C.T., Ravesloot, Wilma T.M., Wijnands, Yvonne, Van Esch, Eric
Format: Article
Language:English
Subjects:
Alpha-Globulins - metabolism
Aniline Compounds
Animals
Biological and medical sciences
Female
Fluorescent Dyes
Hyalin - metabolism
Immunohistochemistry
Kidney - metabolism
Kidney - pathology
Kidney Diseases - chemically induced
Kidney Diseases - metabolism
Kidney Diseases - pathology
Male
Medical sciences
Naphthalenesulfonates
Nephrology. Urinary tract diseases
Nephropathies. Renovascular diseases. Renal failure
Rats
Rats, Wistar
Staining and Labeling - methods
Tubulopathies
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Summary:Hyaline droplet formation in the proximal tubular cells of the kidney commonly occurs under different pathological conditions in experimental animals. In rats, intracytoplasmic hyaline droplet formation is predominantly associated with accumulation of the male rat-specific α2u-globulin, whereas under other (pathological) conditions (eg, histiocytic sarcoma and chronic progressive nephropathy [CPN]) other proteins are involved. Staining methods to visualize hyaline droplets either need plastic embedded material or time-consuming (immuno)histochemical methods. A fast Chromotrope-Aniline-Blue-staining on formalin-fixed paraffin-embedded kidneys taking only 30 minutes is described. Using this method, hyaline droplets consisting of different types of proteins are easily recognized by their bright-red color.
ISSN:0192-6233
1533-1601
DOI:10.1080/01926230390213775