Spermatogenesis in the Cynomolgus Monkey (Macaca fascicularis): A Practical Guide for Routine Morphological Staging

The cynomolgus monkey (Macaca fascicularis) is widely used in regulatory toxicity studies. Especially in studies on male contraception, the male reproductive tract can be an important target system. The aim of the present paper is to describe a practical approach for morphological staging of spermat...

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Bibliographic Details
Published in:Toxicologic pathology 2007-04, Vol.35 (3), p.395-404
Main Authors: Dreef, Henriette C., van Esch, Eric, De Rijk, Eveline P. C. T.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Eosine Yellowish-(YS) - metabolism
Fixatives
Guidelines as Topic
Hematoxylin - metabolism
Histocytochemistry - methods
Macaca fascicularis - physiology
Male
Medical sciences
Paraffin Embedding
Periodic Acid-Schiff Reaction - methods
Seminiferous Tubules - cytology
Seminiferous Tubules - metabolism
Sertoli Cells - cytology
Sertoli Cells - metabolism
Spermatogenesis - physiology
Spermatogonia - cytology
Spermatogonia - metabolism
Spermatozoa - cytology
Spermatozoa - metabolism
Staining and Labeling - methods
Testis - cytology
Testis - metabolism
Toxicology
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Summary:The cynomolgus monkey (Macaca fascicularis) is widely used in regulatory toxicity studies. Especially in studies on male contraception, the male reproductive tract can be an important target system. The aim of the present paper is to describe a practical approach for morphological staging of spermatogenesis in routinely prepared paraffin sections. Results obtained using this approach could help to determine possible drug-related effects on spermatogenesis. As a guide to the investigators, photomicrographs of Bouin-fixed, paraffin-embedded and H&E or PAS stained sections from testis tissue are presented to illustrate the twelve successive morphological stages (cell associations) of normal spermatogenesis. Sexually immature or peripubertal monkeys sometimes are included in toxicity studies. Since the morphological features of the testes of such monkeys can be mistaken for treatment-related abnormalities, the morphologic characteristics of these testes are described and discussed briefly.
ISSN:0192-6233
1533-1601
DOI:10.1080/01926230701230346