Use of the Dot-Blot Immunogold Assay to Identify a Proliferative Antigen in the Initial Cells of a Wheat Stem Meristem

We have devised a protocol for the isolation and identification of a proliferative antigen of the initial cells of wheat stem meristems (termed PAI). We have carried out a variety of immunochemical and immunocytochemical methods, using colloidal gold (CG) complexed with monospecific antibodies to PA...

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Bibliographic Details
Published in:Journal of immunoassay (Monticello, N.Y.) N.Y.), 2000-11, Vol.21 (4), p.401-410
Main Authors: Sumaroka, Marina V., Dykman, Lev A., Bogatyrev, Vladimir A., Evseeva, Nina V., Zaitseva, Irina S., Shchyogolev, Sergei Yu, Volodarsky, Alexander D.
Format: Article
Language:English
Subjects:
Antigens - analysis
Cell Division
Dot-Blot Immunoassay
Gold
Immunoblotting - methods
Immunoenzyme Techniques - methods
Immunogold Labeling
Immunoperoxidase Staining
Initial Cells
Meristem - immunology
Plant Stems - immunology
Proliferative Antigen
Stem Meristem
Triticum - immunology
Triticum aestivum
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Summary:We have devised a protocol for the isolation and identification of a proliferative antigen of the initial cells of wheat stem meristems (termed PAI). We have carried out a variety of immunochemical and immunocytochemical methods, using colloidal gold (CG) complexed with monospecific antibodies to PAI as the marker for the detection of PAI. We have been able to determine the effectiveness of immunoaffinity chromatography in isolating PAI from plant tissues and have shown the advantages of CG over enzyme labels for identification of the antigen. Finally, we have obtained a purified preparation of PAI and have determined its molecular mass (∼83 kDa).
ISSN:0197-1522
DOI:10.1080/01971520009349545